Exploratory metabolomics study of the experimental opisthorchiasis in a laboratory animal model (golden hamster, Mesocricetus auratus)

PLoS Neglected Tropical Diseases
Daria A KokovaOleg A Mayboroda

Abstract

Opisthorchiasis is a parasitic infection caused by the liver flukes of the Opisthorchiidae family. Both experimental and epidemiological data strongly support a role of these parasites in the etiology of the hepatobiliary pathologies and an increased risk of intrahepatic cholangiocarcinoma. Understanding a functional link between the infection and hepatobiliary pathologies requires a detailed description a host-parasite interaction on different levels of biological regulation including the metabolic response on the infection. The last one, however, remains practically undocumented. Here we are describing a host response on Opisthorchiidae infection using a metabolomics approach and present the first exploratory metabolomics study of an experimental model of O. felineus infection. We conducted a Nuclear Magnetic Resonance (NMR) based longitudinal metabolomics study involving a cohort of 30 animals with two degrees of infection and a control group. An exploratory analysis shows that the most noticeable trend (30% of total variance) in the data was related to the gender differences. Therefore further analysis was done of each gender group separately applying a multivariate extension of the ANOVA-ASCA (ANOVA simultaneous component ...Continue Reading

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Citations

Jul 28, 2020·BioMed Research International·Vyacheslav A PetrovIrina V Saltykova
Dec 21, 2019·Scientific Reports·Dominic RitlerBritta Lundström-Stadelmann
Sep 8, 2020·Biomedit︠s︡inskai︠a︡ khimii︠a︡·P G LokhovA I Archakov

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Methods Mentioned

BETA
nuclear magnetic resonance
NMR
PCA

Software Mentioned

R
Topspin
ggplot2
gridExtra
caret
cowplot
Rcpm
Matlab
Python
SampleTrack

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