Exploring binding characteristics and the related competition of different protein-bound uremic toxins

Biochimie
Olivier DeltombeSunny Eloot

Abstract

Little is known about potential differences in binding characteristics of protein-bound uremic toxins (PBUTs) in patients with chronic kidney disease (CKD) versus healthy controls. The question arises whether eventual differences are attributed to (i) the elevated levels of competing uremic toxins, and/or (ii) post-translational modifications of albumin. We evaluated the binding characteristics of hippuric acid (HA), indole-3-acetic acid (IAA), indoxyl sulfate (IS), and p-cresylsulfate (pCS) by deriving a binding curve in three distinct conditions: (i) serum from healthy controls (healthy serum), (ii) blank serum from hemodialysis patients (blank HD serum; i.e. cleared from uremic toxins), and (iii) non-treated serum from HD patients (HD serum). Additionally, the mutual binding competition of these uremic toxins was studied in blank HD in pairs. In both experiments, equilibrium dialysis (37 °C, 5 h) was used to separate the free and bound fractions of each PBUT. Free and total PBUT concentrations were quantified by an ultra-high performance liquid chromatography method with tandem mass spectrometer detection and the percentage protein binding (%PB) of each PBUT was calculated. For all four compounds, the binding capacity of hea...Continue Reading

Citations

Oct 7, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Sigurd DelangheMarijn M Speeckaert
Nov 23, 2018·International Heart Journal·Ippei WatanabeToshisuke Morita
Jul 4, 2019·Toxins·Olivier DeltombeSunny Eloot
Jun 28, 2019·Nature Reviews. Nephrology·Björn MeijersHans-Joachim Anders
Feb 28, 2020·International Journal of Molecular Sciences·Joosep PaatsAlberto Arduan Ortiz
Oct 21, 2020·Nutrients·Sharmela SahathevanTilakavati Karupaiah
Nov 3, 2020·The International Journal of Artificial Organs·Yue ShenFeng Ding
Dec 18, 2020·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Yan ChenUNKNOWN CRIC Study Investigators
Feb 10, 2021·Toxins·Suguru YamamotoIchiei Narita
Dec 22, 2019·Colloids and Surfaces. B, Biointerfaces·Yue ShenFeng Ding
Aug 20, 2021·Journal of the American Society of Nephrology : JASN·Robert D MairTimothy W Meyer

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