Exploring enantiospecific ligand-protein interactions using cellular membrane affinity chromatography: chiral recognition as a dynamic process.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Krzysztof JozwiakIrving William Wainer

Abstract

The chiral recognition mechanisms responsible for the enantioselective binding on the alpha3beta4 nicotinic acetylcholine receptor (alpha3 beta4 nAChR) and human organic cation transporter 1 (hOCT1) have been reviewed. The results indicate that chiral recognition on the alpha3beta4 nAChR is a process involving initial tethering of dextromethorphan and levomethorphan at hydrophobic pockets within the central lumen followed by hydrogen bonding interactions favoring dextromethorphan. The second step is the defining enantioselective step. Studies with the hOCT1 indentified four binding sites within the transporter that participated in chiral recognition. Each of the enantiomers of the compounds used in the study interacted with three of these sites, while (R)-verapamil interacted with all four. Chiral recognition arose from the conformational adjustments required to produce optimum interactions. With respect to the prevailing interaction-based models, the data suggest that chiral recognition is a dynamic process and that the static point-based models should be amended to reflect this.

Citations

Aug 21, 2013·Journal of Pharmaceutical and Biomedical Analysis·Marcela C de MoraesQuezia B Cass
Aug 11, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kanji Miyabe, Satoshi Kamiya
Sep 6, 2012·Journal of Chemical Information and Modeling·N J GumedeS Sagrado
Mar 4, 2017·Biomacromolecules·Yukihiro OkamotoHiroshi Umakoshi

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