Exploring glucosinolates diversity in Brassicaceae: a genomic and chemical assessment for deciphering abiotic stress tolerance

Plant Physiology and Biochemistry : PPB
Anyse Pereira EssohMaria Manuel Romeiras

Abstract

Brassica is one of the most economically important genus of the Brassicaceae family, encompassing several key crops like Brassica napus (cabbage) and broccoli (Brassica oleraceae var. italica). This family is well known for their high content of characteristic secondary metabolites such as glucosinolates (GLS) compounds, recognize for their beneficial health properties and role in plants defense. In this work, we have looked through gene clusters involved in the biosynthesis of GLS, by combining genomic analysis with biochemical pathways and chemical diversity assessment. A total of 101 Brassicaceae genes involved in GLS biosynthesis were identified, using a multi-database approach. Through a UPGMA and PCA analysis on the 101 GLS genes recorded, revealed a separation between the genes mainly involved in GLS core structure synthesis and genes belonging to the CYP450s and MYBs gene families. After, a detailed phylogenetic analysis was conducted to better understand the disjunction of the aliphatic and indolic genes, by focusing on CYP79F1-F2 and CYP81F1-F4, respectively. Our results point to a recent diversification of the aliphatic CYP79F1 and F2 genes in Brassica crops, while for indolic genes an earliest diversification is obs...Continue Reading

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