Exploring maternal serum microRNAs during early pregnancy in cattle

Theriogenology
Samuel GebremedhnDawit Tesfaye

Abstract

Confirmation of the pregnancy establishment at the very earliest day post-insemination increases the reproduction efficiency of high yielding dairy cows and farm profitability by allowing rebreeding of the non-pregnant cows. Inaccuracies in the currently available pregnancy detection tools to detect pregnancy establishment within the first 3 weeks post insemination extends the inter-calving interval and have contributed to the decline in profitability. Thus, development of non-invasive early pregnancy detection biomarkers could be proposed as alternative tools. MicroRNAs (miRNAs), a subclass of small non-coding RNAs are abundantly expressed in virtually all bio fluids circulation and have been associated with various pregnancy-related pathophysiological conditions. The study aimed to determine the expression of circulatory miRNAs in serum samples of pregnant and non-pregnant cows at day 19 and 24 post-insemination. Lactating Holstein-Friesian cows were estrous synchronized and inseminated with frozen semen. Blood samples were taken 19 and 24 days post-insemination. Serum samples were retrospectively categorized according to the pregnancy status of cows diagnosed 35 later using ultrasonography. Total RNA enriched with miRNAs was...Continue Reading

Citations

Oct 8, 2019·Journal of Dairy Science·Alan D Ealy, Zachary K Seekford
Jan 21, 2020·Cancer Science·Jiajia LiuXin Jin
Oct 9, 2020·Animal Reproduction·Ky Garrett PohlerRebecca Kyle Poole
Mar 7, 2021·International Journal of Molecular Sciences·Natalie TurnerMurray D Mitchell

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