Exploring membrane domains using native membrane sheets and transmission electron microscopy

Methods in Molecular Biology
B S WilsonJanet M Oliver

Abstract

The flow of information in cells requires the constant remodeling of cell signaling and trafficking networks. To observe the remodeling events associated with activation of receptors on the cell surface, the authors have generated and analyzed high-resolution topographical maps of colloidal gold nanoprobes (3-10 nm) marking receptors, signaling proteins, and lipids in native membranes. The technology involves sandwiching of cells between glass cover slips and electron microscopy (EM) grids, followed by ripping. Membrane sheets on EM grids are fixed, labeled with functionalized nanoprobes, and imaged by transmission electron microscopy. Probe coordinates are extracted from digitized images and the distributions of the probes are analyzed with respect to each other and to membrane features like clathrin-coated pits, caveolae, and the cortical cytoskeleton.

Citations

Jun 7, 2012·Annals of Biomedical Engineering·Krishnan RadhakrishnanBridget S Wilson
Jul 14, 2011·Bulletin of Mathematical Biology·Flor A EspinozaStanly L Steinberg
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tom Li StephenTerri M Laufer
Dec 29, 2010·The Journal of Cell Biology·Allen P LiuSandra L Schmid
Mar 17, 2010·The Journal of Cell Biology·Marcel MettlenSandra L Schmid
Feb 17, 2017·Journal of Virology·George P Leser, Robert A Lamb
Oct 6, 2010·FEBS Letters·Jianying Yang, Michael Reth
Jan 6, 2012·American Journal of Physiology. Renal Physiology·A PaliegeS Bachmann
Jan 1, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amanda Carroll-PortilloJerilyn A Timlin
Aug 30, 2013·Journal of Cell Science·Cédric CleyratBridget S Wilson

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