Exploring membrane selectivity of the antimicrobial peptide KIGAKI using solid-state NMR spectroscopy

Biochimica Et Biophysica Acta
Jun-Xia LuGary A Lorigan

Abstract

The designed antimicrobial peptide KIGAKIKIGAKIKIGAKI possesses enhanced membrane selectivity for bacterial lipids, such as phosphatidylethanolamine and phosphatidylglycerol. The perturbation of the bilayer by the peptide was first monitored using oriented bilayer samples on glass plates. The alignment of POPE/POPG model membranes with respect to the bilayer normal was severely altered at 4 mol% KIGAKI while the alignment of POPC bilayers was retained. The interaction mechanism between the peptide and POPE/POPG bilayers was investigated by carefully comparing three bilayer MLV samples (POPE bilayers, POPG bilayers, and POPE/POPG 4/1 bilayers). KIGAKI induces the formation of an isotropic phase for POPE/POPG bilayers, but only a slight change in the (31)P NMR CSA line shape for both POPE and POPG bilayers, indicating the synergistic roles of POPE and POPG lipids in the disruption of the membrane structure by KIGAKI. (2)H NMR powder spectra show no reduction of the lipid chain order for both POPG and POPE/POPG bilayers upon peptide incorporation, supporting the evidence that the peptide acts as a surface peptide. (31)P longitudinal relaxation studies confirmed that different dynamic changes occurred upon interaction of the peptid...Continue Reading

References

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Citations

Apr 24, 2009·Solid State Nuclear Magnetic Resonance·Ayyalusamy Ramamoorthy
Sep 21, 2010·Journal of Magnetic Resonance·Shidong ChuGary A Lorigan
Dec 15, 2007·Biochimica Et Biophysica Acta·David W Hoskin, Ayyalusamy Ramamoorthy
Feb 25, 2017·Magnetic Resonance in Chemistry : MRC·Rongfu ZhangGary A Lorigan
Mar 16, 2018·Journal of Peptide Science : an Official Publication of the European Peptide Society·Hui Ye
Jul 10, 2018·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Sabine ReißerAnne S Ulrich
Mar 29, 2012·Journal of the American Chemical Society·Parvesh WadhwaniAnne S Ulrich
Jan 7, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Tânia SilvaMargarida Bastos

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