Exploring methods to expedite the recording of CEST datasets using selective pulse excitation

Journal of Magnetic Resonance
Tairan YuwenTairan Yuwen

Abstract

Chemical Exchange Saturation Transfer (CEST) has emerged as a powerful tool for studies of biomolecular conformational exchange involving the interconversion between a major, visible conformer and one or more minor, invisible states. Applications typically entail recording a large number of 2D datasets, each of which differs in the position of a weak radio frequency field, so as to generate a CEST profile for each nucleus from which the chemical shifts of spins in the invisible state(s) are obtained. Here we compare a number of band-selective CEST schemes for speeding up the process using either DANTE or cosine-modulated excitation approaches. We show that while both are essentially identical for applications such as 15N CEST, in cases where the probed spins are dipolar or scalar coupled to other like spins there can be advantages for the cosine-excitation scheme.

Citations

Apr 18, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Tairan YuwenGuillaume Bouvignies
Jan 21, 2019·Journal of Biomolecular NMR·Ved Prakash TiwariPramodh Vallurupalli
Jul 23, 2020·Journal of Biomolecular NMR·Ved Prakash Tiwari, Pramodh Vallurupalli
Mar 5, 2020·Progress in Nuclear Magnetic Resonance Spectroscopy·Stefan Schütz, Remco Sprangers
Dec 14, 2019·Current Opinion in Structural Biology·T Reid Alderson, Lewis E Kay
Sep 29, 2019·Journal of the American Chemical Society·Gregory JamesonRafael Brüschweiler
May 14, 2019·The Journal of Physical Chemistry Letters·Pramodh VallurupalliShamasree Ghosh

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