Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS

Toxins
Annika JagelsHans-Ulrich Humpf

Abstract

The genus Stachybotrys produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structural modifications, few investigations have been carried out. Thus, the presented study deals with the quantitative determination of several secondary metabolites produced by distinct Stachybotrys species for comparison of their metabolite profiles. For that purpose, 15 of the primarily produced secondary metabolites were isolated from fungal cultures and structurally characterized in order to be used as analytical standards for the development of an LC-MS/MS multimethod. The developed method was applied to the analysis of micro-scale extracts from 5 different Stachybotrys strains, which were cultured on different media. In that process, spontaneous dialdehyde/lactone isomerization was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes were quantitatively investigated for the first time. The metabolite profiles of Stachybotrys species are considerably influenced by time of growth and substrate availability, as well as ...Continue Reading

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Citations

Sep 6, 2020·Journal of Fungi·Sebastian Ulrich, Cornelius Schäfer
Jun 24, 2021·Nature Communications·Robin SchmidPieter C Dorrestein
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Samer HaidarJoachim Jose

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Methods Mentioned

BETA
NMR
deamination
column chromatography

Software Mentioned

Analyst
STDIAL

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