Exploring stemness gene expression and vasculogenic mimicry capacity in well- and poorly-differentiated hepatocellular carcinoma cell lines

Biochemical and Biophysical Research Communications
Kriengsak LirdprapamongkolJisnuson Svasti

Abstract

Vasculogenic mimicry (VM) is the phenomenon where cancer cells mimic endothelial cells by forming blood vessels. A stem cell-like phenotype has been proposed to be involved in this tumor plasticity. VM seems to correlate with metastasis rate, but there have been no reports on the effects of pro-metastatic and pro-angiogenic factors or hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) on VM formation of hepatocellular carcinoma (HCC) cells. Here, we determine VM capacity and expression of stemness genes (Oct4, Sox2, Nanog and CD133) in well- and poorly-differentiated HCC cell lines. The poorly-differentiated cell line SK-Hep-1 with mesenchymal features (high invasiveness and expressing Vimentin, with no E-cadherin) could form VM in vitro, while the well-differentiated cell line HepG2 did not form VM. There was no correlation between expression of stemness genes and intrinsic VM capacity. However, HGF but not VEGF, could induce VM formation in HepG2, concomitant with epithelial-mesenchymal transition (EMT), de-differentiation and increased expression of stemness genes. Our results show that the role of stemness genes in VM capacity of HCC cells is likely to depend on differentiation status.

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Citations

Sep 24, 2015·Biochemical and Biophysical Research Communications·Yesol BakDae-Yeul Yu
Mar 31, 2016·Journal of Cellular and Molecular Medicine·Qiqi LiuJiandong Zhang
Sep 17, 2015·Biochemical and Biophysical Research Communications·Korakot AtjanasuppatJisnuson Svasti
Jan 20, 2015·Journal of Cellular and Molecular Medicine·Lili QiaoYuan Tian
Sep 3, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Zhifei CaoQuansheng Zhou
Dec 17, 2015·Journal of Cellular Biochemistry·Pedro CoelhoRaquel Soares
Aug 24, 2013·Oncology Reports·Kriengsak LirdprapamongkolIkuo Saiki
Oct 15, 2019·International Journal of Cancer. Journal International Du Cancer·Jeerawan KlangjorhorDumnoensun Pruksakorn
Mar 23, 2017·Molecular Cancer·Daniel Delgado-BellidoF Javier Oliver
May 28, 2019·International Journal of Molecular Sciences·Shinsuke KikuchiTakahiro Ochiya
Nov 2, 2013·Oncology Letters·Yun-Long FanXin-Hua Liang
Feb 22, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ning ZhengJichuang Wang
Jun 1, 2014·Disease Models & Mechanisms·Marco SchiavoneFrancesco Argenton

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