Abstract
Cytochrome bo-type ubiquinol oxidase is a four-subunit heme-copper terminal oxidase and functions as a redox-coupled proton pump in the aerobic respiratory chain of Escherichia coli. On the basis of deletion and chemical cross-linking analyses on subunit IV, we proposed that subunit IV is essential for CuB binding to subunit I and that it is present in a cleft between subunits I and III (Saiki, K., Nakamura, H., Mogi, T., and Anraku, Y. (1996) J. Biol. Chem. 271, 15336-15340). To extend previous studies, we carried out alanine-scanning mutagenesis for selected 16-amino acid residues in subunit IV to explore subunit-subunit interactions in bo-type ubiquinol oxidase. We found that only the replacement of Phe83 in helix III resulted in the reduction of the catalytic activity but that this did not significantly affect the UV-visible spectroscopic properties and the copper content. This suggests that individual amino acid substitutions, including the six invariant residues, are not enough to alter such properties of the metal centers. Extragenic suppressor mutations were isolated for the Phe83 --> Ala mutation of subunit IV and identified as missense mutations in helices VII and VIII in subunit I. These observations provide further ...Continue Reading
References
Nov 1, 1990·Quarterly Reviews of Biophysics·M Saraste
Jun 5, 1989·FEBS Letters·A PuustinenM Wikström
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K MatsushitaH R Kaback
Jul 10, 1995·FEBS Letters·D M Mitchell, R B Gennis
Aug 24, 1995·Nature·S IwataH Michel
Aug 21, 1995·FEBS Letters·T MogiY Orii
Sep 1, 1994·Journal of Biochemistry·T MogiY Anraku
Nov 1, 1994·Molecular Microbiology·T MogiY Anraku
Sep 1, 1994·Journal of Bacteriology·J A García-HorsmanR B Gennis
May 13, 1994·Science·S W Cowan, J P Rosenbusch
Jun 15, 1993·Biochemistry·M TsubakiH Hori
Dec 1, 1995·European Journal of Biochemistry·A WarneM Saraste
Jan 12, 1996·Science·D A Dougherty
Feb 15, 1996·Biochimica Et Biophysica Acta·T KusanoN Sone
May 24, 1996·Science·T TsukiharaS Yoshikawa
Jun 28, 1996·The Journal of Biological Chemistry·K SaikiY Anraku
Jul 1, 1996·Nature Structural Biology·R A Capaldi
Jul 1, 1950·Journal of Bacteriology·B D DAVIS, E S MINGIOLI
Citations
May 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J ChenG I Henderson
Jan 3, 2001·Journal of Bacteriology·M SantanaM Teixeira
Feb 20, 2010·BMC Microbiology·Farahnaz MovahedzadehNeil G Stoker
May 16, 1998·Biochemistry·M Sato-WatanabeY Anraku
Aug 1, 2009·EcoSal Plus·Vitaliy B Borisov, Michael I Verkhovsky
Jan 7, 2016·EcoSal Plus·Vitaliy B Borisov, Michael I Verkhovsky
Sep 16, 1998·Biochemistry·M Sato-WatanabeY Anraku
Jun 1, 2000·Biochemistry·A PrutschM Lübben
Dec 12, 2000·Biochemistry·K KobayashiT Mogi
Nov 24, 1999·Biochemistry·J MaR B Gennis