Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium.

Genome Biology
Yoshinori TomoyasuGregor Bucher

Abstract

RNA interference (RNAi) is a highly conserved cellular mechanism. In some organisms, such as Caenorhabditis elegans, the RNAi response can be transmitted systemically. Some insects also exhibit a systemic RNAi response. However, Drosophila, the leading insect model organism, does not show a robust systemic RNAi response, necessitating another model system to study the molecular mechanism of systemic RNAi in insects. We used Tribolium, which exhibits robust systemic RNAi, as an alternative model system. We have identified the core RNAi genes, as well as genes potentially involved in systemic RNAi, from the Tribolium genome. Both phylogenetic and functional analyses suggest that Tribolium has a somewhat larger inventory of core component genes than Drosophila, perhaps allowing a more sensitive response to double-stranded RNA (dsRNA). We also identified three Tribolium homologs of C. elegans sid-1, which encodes a possible dsRNA channel. However, detailed sequence analysis has revealed that these Tribolium homologs share more identity with another C. elegans gene, tag-130. We analyzed tag-130 mutants, and found that this gene does not have a function in systemic RNAi in C. elegans. Likewise, the Tribolium sid-like genes do not see...Continue Reading

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Datasets Mentioned

BETA
EF695396
EF695398
EF688531
EF688530
EF688527
EF688528
EF688529
EU273915
AB327183
AB327184

Methods Mentioned

BETA
gene knockdown
PCR
two-hybrid
in

Software Mentioned

tBLASTn
matcher
PROSITE
ClustalW
BLAST
BLASTx
MEGA
TMHMM
InterProScan
KAIKObase

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