Exploring the catalytic mechanism of a novel β-glucosidase BGL0224 from Oenococcus oeni SD-2a: Kinetics, spectroscopic and molecular simulation.

Enzyme and Microbial Technology
Jie ZhangMingtao Fan

Abstract

The β-glucosidase derived from microorganisms has attracted worldwide interest for their industrial applications, but studies on β-glucosidases from Oenococcus oeni are rare. In this paper, catalytic mechanism of a novel β-glucosidase BGL0224 of Oenococcus oeni SD-2a was explored for the first time by kinetic parameters determination, fluorescence spectroscopy and quenching mechanism analysis, molecular dynamics simulation. The results indicated that BGL0224 had universal catalytic effect on different types of glycoside substrates, but the catalytic efficiencies were different. Fluorescence quenching analysis results suggested that the quenching processes between BGL0224 and seven kinds of substrates were predominated by the static quenching mechanism. A reasonable three-dimensional model of BGL0224 was obtained using the crystal structure of E.coli BglA as a template. The analysis results of molecular simulation (RMSD, Rg, RMSF and hydrogen bonding) showed that the composite system 'BGL0224-pNPG' was very stable after 40 ns. The catalytic process of BGL0224 acting on 'p-Nitrophenyl β-d-glucopyranoside' conformed to the double displacement mechanism. Two glutamic acid residues 'Glu178 and Glu377' played a vital role in the whol...Continue Reading

References

Feb 1, 1977·Canadian Journal of Microbiology·D SternbergE T Reese
Mar 1, 1990·Journal of Computer-aided Molecular Design·J J Stewart
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·D Leckband
Dec 19, 2002·Critical Reviews in Biotechnology·Yukti BhatiaV S Bisaria
Mar 14, 2003·Physical Review Letters·R MartonákM Parrinello
Oct 8, 2005·Journal of Computational Chemistry·David Van Der SpoelHerman J C Berendsen
Jul 8, 2008·Protein Expression and Purification·Piyanun HarnpicharnchaiLily Eurwilaichitr
Nov 27, 2009·The Journal of Biological Chemistry·Abigail I GuceScott C Garman
May 22, 2010·Cellular and Molecular Life Sciences : CMLS·James R Ketudat Cairns, Asim Esen
Jun 14, 2013·The Journal of General and Applied Microbiology·Cristiane Akemi UchimaManabu Arioka
Jul 13, 2013·Journal of Biomolecular Structure & Dynamics·Mahesh GokaraRajagopal Subramanyam
Feb 28, 2014·Methods in Molecular Biology·Benjamin Webb, Andrej Sali
Aug 27, 2014·Organic & Biomolecular Chemistry·Kah-Yee LiHerman S Overkleeft
Mar 30, 2017·Analytical Chemistry·Christopher J GraySabine L Flitsch
Jan 10, 2018·Enzyme and Microbial Technology·Perla Guadalupe Vazquez-OrtegaRoberto Fernandez-Lafuente
Nov 26, 2019·International Journal of Biological Macromolecules·Jian-Li LiuXiang-Yu Cao

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