Abstract
The detoxification from peroxides in Sulfolobus solfataricus is performed by the Bacterioferritin comigratory proteins (Bcps), Bcp1 (Sso2071), Bcp2 (Sso2121), Bcp3 (Sso2255) and Bcp4 (Sso2613), antioxidant enzymes belonging to one of the subfamilies of the Peroxiredoxins. In this paper we report on the functional, structural and docking analyses of Bcp4, characterized by the CXXXXC motif in the active site. Bcp4 represents the first dimeric Bcp so far investigated. Biochemical studies showed that the protein has a non-covalent dimeric structure and adopts an atypical 2-Cys catalytic mechanism. The X-ray structure of the double mutant C45S/C50S, representative of the fully reduced enzyme state, described the protein dimeric arrangement. Finally, concurrent availability of the crystallographic structure of the monomeric Bcp1 allowed comparative analysis of the interaction with Protein Disulfide Oxidoreductase SsPDO (Sso0192), involved in the reduction of both Bcp1 and Bcp4, through a protein-protein docking approach.
References
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Apr 20, 1990·European Journal of Biochemistry·A McPherson
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 20, 1998·Nature Structural Biology·H J ChoiS E Ryu
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jan 25, 2000·The Journal of Biological Chemistry·W JeongI H Kim
Apr 24, 2002·Biochemistry·Zachary A WoodP Andrew Karplus
Apr 23, 2003·The Journal of Biological Chemistry·Sung-Jong Jeon, Kazuhiko Ishikawa
Jun 5, 2003·Proteins·Rong ChenZhiping Weng
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch
Aug 2, 2003·Nature·Carola A NeumannRichard A Van Etten
Oct 7, 2003·Annual Review of Microbiology·James A Imlay
Feb 21, 2004·Plant Physiology·Nicolas RouhierJean-Pierre Jacquot
Jul 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·R CudneyA McPherson
Feb 11, 2005·Journal of Molecular Biology·Ganapathy N SarmaP Andrew Karplus
Feb 11, 2005·Journal of Molecular Biology·Simon LiEdward N Baker
Oct 26, 2005·Proteins·Jongkeun ChoiWhanchul Shin
Jan 31, 2006·The FEBS Journal·Danila LimauroSimonetta Bartolucci
Aug 4, 2006·Journal of Molecular Biology·Beom-Seop RhoJean-Denis Pédelacq
Aug 29, 2006·Journal of Molecular Biology·Katia D'AmbrosioSimonetta Bartolucci
Apr 28, 2007·Free Radical Biology & Medicine·Mee-Kyung ChaIl-Han Kim
May 29, 2007·Journal of Molecular Biology·Cha San KohCatherine Corbier
Jul 24, 2007·The Journal of Biological Chemistry·Masahiro WakitaKen-ichiro Takamiya
Mar 22, 2008·The FEBS Journal·Danila LimauroSimonetta Bartolucci
Jun 12, 2008·Free Radical Biology & Medicine·Christine C Winterbourn, Mark B Hampton
Nov 13, 2008·Bioinformatics·Christopher ReynoldsSusan Jones
Mar 21, 2009·Biochemistry·David J ClarkeAlan R Brown
Apr 2, 2009·Proteins·Katia D'AmbrosioGiuseppina De Simone
Citations
Aug 30, 2012·Biochemistry·Arden PerkinsP Andrew Karplus
Dec 7, 2013·Extremophiles : Life Under Extreme Conditions·Danila LimauroEmilia Pedone
Oct 19, 2016·Archaea : an International Microbiological Journal·Carmen SarcinelliDanila Limauro
Mar 3, 2017·Extremophiles : Life Under Extreme Conditions·Jae Kyu LimHyun Sook Lee
Apr 11, 2018·Antioxidants & Redox Signaling·David YoungJoris Messens
Feb 14, 2018·PloS One·Tao SuCan Chen
Nov 30, 2018·FEBS Letters·Chuloh ChoSeung Sik Lee
Jun 18, 2019·Acta Crystallographica. Section F, Structural Biology Communications·Sander StroobantsEveline Peeters
May 10, 2020·Antioxidants·Mamta Rawat, Julie A Maupin-Furlow
Nov 28, 2017·Extremophiles : Life Under Extreme Conditions·Francesca Anna FuscoDanila Limauro
Aug 7, 2020·Antioxidants·Emilia PedoneDanila Limauro
Feb 15, 2017·Microbial Cell Factories·Martina AulittoPatrizia Contursi
Mar 9, 2018·International Journal of Biological Macromolecules·Francesca Anna FuscoDanila Limauro
Nov 2, 2013·Biochemistry·Arden PerkinsP Andrew Karplus
Jul 1, 2018·New Biotechnology·Mariamichela LanzilliRachele Isticato