Exploring the dihydrodipicolinate synthase tetramer: how resilient is the dimer-dimer interface?

Archives of Biochemistry and Biophysics
Michael D W GriffinMatthew A Perugini

Abstract

Dihydrodipicolinate synthase (DHDPS, E.C. 4.2.1.52) is a tetrameric enzyme that catalyses the first committed step of the lysine biosynthetic pathway. Dimeric variants of DHDPS have impaired catalytic activity due to aberrant protein motions within the dimer unit. Thus, it is thought that the tetrameric structure functions to restrict these motions and optimise enzyme dynamics for catalysis. Despite the importance of dimer-dimer association, the interface between subunits of each dimer is small, accounting for only 4.3% of the total monomer surface area, and the structure of the interface is not conserved across species. We have probed the tolerance of dimer-dimer association to mutation by introducing amino acid substitutions within the interface. All point mutations resulted in destabilisation of the 'dimer of dimers' tetrameric structure. Both the position of the mutation in the interface and the physico-chemical nature of the substitution appeared to effect tetramerisation. Despite only weak destabilisation of the tetramer by some mutations, catalytic activity was reduced to approximately 10-15% of the wild-type in all cases, suggesting that the dimer-dimer interface is finely tuned to optimise function.

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Citations

Nov 4, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jacinta M WubbenMatthew A Perugini
Nov 22, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Michael D W GriffinF Grant Pearce
Dec 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sarah C AtkinsonMatthew A Perugini
Jul 14, 2012·PloS One·Michael D W GriffinFrederick Grant Pearce
Mar 29, 2014·Proteins·Sarah C AtkinsonMatthew A Perugini
Jul 19, 2016·Structure·Tatiana P Soares da CostaMatthew A Perugini
Nov 16, 2016·Scientific Reports·Janni B ChristensenMatthew A Perugini
Oct 9, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tanzeela SiddiquiMatthew A Perugini
Apr 18, 2020·Molecular Biology and Evolution·Ashar J MalikJane R Allison
Dec 6, 2017·Biophysics Reviews·Tatiana P Soares da CostaMatthew A Perugini
May 29, 2018·Structure·Sarah C AtkinsonMatthew A Perugini
Jun 28, 2011·Archives of Biochemistry and Biophysics·Sudhir R DommarajuMatthew A Perugini

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