Exploring the effect of vitamin E in cancer chemotherapy-A biochemical and biophysical insight

Journal of Biophotonics
Mustansir BhoriMurali Krishna Chilakapati

Abstract

Many oncologists contend that patient undergoing chemotherapy must avoid antioxidant supplementation as it may interfere with the activity of the drug. In the present investigation, we have explored the influence of vitamin E, a well-known antioxidant on Camptothecin (CPT), a potent anti-cancer drug induced cell apoptosis and death of cervical cancer cells. HeLa cells were treated with different concentrations of CPT in presence and absence of 100 μm vitamin E. Treated cells were subjected to cytotoxicity studies, catalase assay, DNA fragmentation assay, clonogenic assay and flow cytometry based apoptosis detection. Also, Raman spectroscopy a label free technique which provides global information, in conjunction with multivariate tools like PCA, PCLDA and FDA, was investigated to explore vitamin E supplementation induced alterations. Our data based on biochemical and biophysical experimental analysis reveals that CPT causes DNA damage along with protein and lipid alteration culminating in cell death. Importantly, Raman spectroscopic analysis could uniquely differentiate the cluster of control and vitamin E control from CPT and CPT + Vit E treated cells. We conclusively prove that presence of vitamin E at 100 μM concentration sh...Continue Reading

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Citations

Oct 1, 2019·Journal of Biophotonics·Kshama PansarePoonam Gera
Feb 8, 2020·Applied Spectroscopy·Kshama PansareChilakapati Murali Krishna
Nov 4, 2020·Nutrients·Julia Kaźmierczak-BarańskaBoleslaw T Karwowski

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