Exploring the effects of the atherosclerosis progression and the choice of affected arteries in the design of experiments with Apolipoprotein E-deficient mice

Clínica e investigación en arteriosclerosis : publicación oficial de la Sociedad Española de Arteriosclerosis
Marta Riera-BorrullJorge Joven

Abstract

The objective of this study is to explore the longitudinal progression of atherosclerosis and the correlation between methods to measure the lesion in apolipoprotein E-deficient mice. Atherosclerosis progression was assessed by measurements of foam cell-rich depositions in their proximal aortas, and/or in surgically excised arteries, to assess the histological luminal narrowing. A longitudinal study was performed by comparing the values for carotid, aorta, and femoral and iliac arteries using common histological techniques. There were no significant differences in progression between different arteries, but correlation with the classical measurement of atherosclerosis in the aortic root was poor. Each laboratory requires specific standardization. Carotid arteries were sensitive to atherosclerosis in these mice, and progression was exponential. In conclusion, morphometric data show the importance of the choice of the duration of treatment, the appropriate controls, and the age at which to begin the experiments.

References

Dec 1, 1987·Atherosclerosis·B PaigenR A Williams
Sep 1, 1994·The Journal of Clinical Investigation·S H ZhangN Maeda
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·S IshibashiM S Brown
May 17, 2005·Methods : a Companion to Methods in Enzymology·Alan Daugherty, Debra L Rateri
Jan 13, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Natàlia FerréJoan Clària
Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kosuke TsukamotoMonty Krieger
Sep 19, 2014·European Journal of Clinical Investigation·Nathan ArtomAldo Pende

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