Exploring the electron transfer pathways in photosystem I by high-time-resolution electron paramagnetic resonance: observation of the B-side radical pair P700(+)A1B(-) in whole cells of the deuterated green alga Chlamydomonas reinhardtii at cryogenic temperatures

Journal of the American Chemical Society
T BertholdGerd Kothe

Abstract

Crystallographic models of photosystem I (PS I) highlight a symmetrical arrangement of the electron transfer cofactors which are organized in two parallel branches (A, B) relative to a pseudo-C2 symmetry axis that is perpendicular to the membrane plane. Here, we explore the electron transfer pathways of PS I in whole cells of the deuterated green alga Chlamydomonas reinhardtii using high-time-resolution electron paramagnetic resonance (EPR) at cryogenic temperatures. Particular emphasis is given to quantum oscillations detectable in the tertiary radical pairs P700(+)A1A(-) and P700(+)A1B(-) of the electron transfer chain. Results are presented first for the deuterated site-directed mutant PsaA-M684H in which electron transfer beyond the primary electron acceptor A0A on the PsaA branch of electron transfer is impaired. Analysis of the quantum oscillations, observed in a two-dimensional Q-band (34 GHz) EPR experiment, provides the geometry of the B-side radical pair. The orientation of the g tensor of P700(+) in an external reference system is adapted from a time-resolved multifrequency EPR study of deuterated and 15N-substituted cyanobacteria (Link, G.; Berthold, T.; Bechtold, M.; Weidner, J.-U.; Ohmes, E.; Tang, J.; Poluektov, ...Continue Reading

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Citations

Feb 7, 2015·Biochimica Et Biophysica Acta·Michael D McConnellArt van der Est
Mar 22, 2016·Physical Chemistry Chemical Physics : PCCP·Stefano Santabarbara, Giuseppe Zucchelli
Jun 7, 2013·Metallomics : Integrated Biometal Science·Inmaculada Yruela
Jul 18, 2019·Frontiers in Plant Science·Stefano Santabarbara, Anna Paola Casazza
May 25, 2020·Photosynthesis Research·Neva AgarwalaGary Hastings
Aug 14, 2019·The Journal of Physical Chemistry. B·Oleg G PoluektovLisa M Utschig

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