Exploring the interfacial structure of protein adsorbates and the kinetics of protein adsorption: an in situ high-energy X-ray reflectivity study

Langmuir : the ACS Journal of Surfaces and Colloids
Florian EversMetin Tolan

Abstract

The high energy X-ray reflectivity technique has been applied to study the interfacial structure of protein adsorbates and protein adsorption kinetics in situ. For this purpose, the adsorption of lysozyme at the hydrophilic silica-water interface has been chosen as a model system. The structure of adsorbed lysozyme layers was probed for various aqueous solution conditions. The effect of solution pH and lysozyme concentration on the interfacial structure was measured. Monolayer formation was observed for all cases except for the highest concentration. The adsorbed protein layers consist of adsorbed lysozyme molecules with side-on or end-on orientation. By means of time-dependent X-ray reflectivity scans, the time-evolution of adsorbed proteins was monitored as well. The results of this study demonstrate the capabilities of in situ X-ray reflectivity experiments on protein adsorbates. The great advantages of this method are the broad wave vector range available and the high time resolution.

References

Dec 16, 1998·Journal of Colloid and Interface Science·T J SuJ Penfold
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·L K Steinrauf
Oct 30, 1998·Journal of Colloid and Interface Science·M Malmsten
Feb 19, 2000·Current Opinion in Structural Biology·A P Minton
Oct 25, 2000·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·A G RichterP Dutta
Mar 27, 2001·Protein Science : a Publication of the Protein Society·D K Eggers, J S Valentine
Sep 19, 2001·Biochemistry·H X Zhou, K A Dill
Jun 1, 2004·Physical Review Letters·S EngemannA Snigirev
Dec 24, 2005·Journal of Synchrotron Radiation·Christof KrywkaMetin Tolan
Feb 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael LefenfeldBenjamin M Ocko
Jul 11, 2006·Journal of Cell Science·Allen P Minton
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Markus MezgerVeijo Honkimäki
Jan 26, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Larry D UnsworthJohn L Brash

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Citations

Sep 14, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Christoph JeworrekRoland Winter
Oct 19, 2011·Physical Chemistry Chemical Physics : PCCP·Deborah WakehamRob Atkin
Feb 25, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Irena KieselMetin Tolan
Oct 20, 2012·Journal of Colloid and Interface Science·Nicolas BrouetteMichele Sferrazza
Nov 7, 2015·The Journal of Physical Chemistry. B·Florian PlattenStefan U Egelhaaf
Sep 1, 2015·Physical Chemistry Chemical Physics : PCCP·Karina Kubiak-OssowskaPaul A Mulheran
Dec 25, 2013·Journal of Synchrotron Radiation·Florian J WirkertMetin Tolan
May 10, 2015·The Journal of Chemical Physics·Florian PlattenStefan U Egelhaaf
Mar 10, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Krassimir Stoev, Kenji Sakurai
Aug 29, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·M W A SkodaM Grunze
Jan 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Martin BrücherRoland Hergenröder
Aug 3, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Antonio M González-DelgadoLuis Camacho
Apr 17, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Yury ForovMetin Tolan
Mar 23, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Carlos Rubia-PayáLuis Camacho
Mar 1, 2018·The Journal of Physical Chemistry. B·Susanne DoganMetin Tolan
Feb 6, 2020·ACS Applied Materials & Interfaces·Ankit D KantheRaymond S Tu
Jun 16, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Brett WalletVladimir V Tsukruk
Apr 27, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Hendrik HählKarin Jacobs
Apr 17, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Andrew G Richter, Ivan Kuzmenko

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