Exploring the Microbiota of Diabetic Foot Infections With Culturomics

Frontiers in Cellular and Infection Microbiology
Joanne JneidBernard La Scola

Abstract

The purpose of this prospective observational study was to evaluate the richness and diversity of bacteria in samples from diabetic foot infections using a culturomics approach. Bacterial culture findings from wound samples were analyzed together with clinical characteristics and treatment outcomes. We included 43 patients admitted to a French referral center with a moderate to severe diabetic foot infection. The 30,000 colonies identified yielded 53 different bacterial species. The global α-Shannon diversity was 3.34 and the bacterial richness per patient was 4 ± 2. Of all the identified bacterial species, 19 (35.8%) had never been previously cultured or identified by molecular methods from diabetic foot ulcers. Most of the samples were polymicrobial (N = 38; 88.3%). Of all the isolated species, the most prevalent were Staphylococcus aureus (N = 28; 52.8%), Enterococcus faecalis (N = 24; 45.2%), Enterobacter cloacae (N = 12; 22.6%), Staphylococcus lugdunensis (N = 10; 18.7%), Staphylococcus epidermidis (N = 6; 11.3%), Proteus mirabilis (N = 6; 11.3%), and Finegoldia magna (N = 5; 9.4%). The only factor associated with wound improvement after a 1-month follow-up was the presence of E. faecalis (p = 0.012) when compared with pat...Continue Reading

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Citations

Dec 7, 2018·Expert Review of Anti-infective Therapy·Silvano EspositoPasquale Pagliano
Jul 16, 2019·Expert Review of Molecular Diagnostics·Markus KostrzewaArthur B Pranada
Jul 19, 2019·Clinical Microbiology Reviews·Anne Davin-RegliJean-Marie Pagès
Feb 14, 2020·Expert Review of Anti-infective Therapy·Bülent ErtuğrulBenjamin A Lipsky
Mar 6, 2019·Frontiers in Cellular and Infection Microbiology·Thaysa Leite TagliaferriHans-Peter Horz
Nov 14, 2019·Journal of Clinical Medicine·Fatemah Sadeghpour HeraviHonghua Hu
May 3, 2020·NPJ Biofilms and Microbiomes·Samuel VerbanicIrene A Chen
Oct 18, 2020·Microorganisms·Cassandra PougetJean-Philippe Lavigne
Nov 25, 2020·BMC Medicine·Chunan LiuBonnie L Hurwitz
Dec 11, 2020·Journal of the American Podiatric Medical Association·Arthur TarriconeRonald L Soave
Nov 15, 2020·Science Advances·Lance R ThurlowAnthony R Richardson
Apr 27, 2021·Frontiers in Immunology·Zoya VerseyEdana Cassol
Aug 14, 2021·Frontiers in Microbiology·Rodrigo Cuiabano Paes Leme, Raquel Bandeira da Silva
Aug 27, 2021·Frontiers in Molecular Biosciences·Jun ChenChun Loong Ho
Sep 18, 2021·Scientific Reports·Catherine Dunyach-RemyAnthony Gélis

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Methods Mentioned

BETA
amputation
PCR
scraping

Software Mentioned

SPSS statistics
XLSTAT

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