Abstract
Restenosis is resulted from the proliferation and migration of vascular smooth muscle cells (VSMCs) from the arterial media into the intima within the vessel lumen following percutaneous transluminal coronary angioplasty (PTCA). OSU-03012, a synthetic compound (2-amino-N-{4-[5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-phenyl} acetamide) acting as a PDK-1 inhibitor, is used as an apoptosis-promoting anticancer drug. However, whether OSU-03012 can inhibit VSMC proliferation and migration following PTCA remains unclear. In this study, we used A10 smooth muscle cells cultured in 10% FBS for stimulating proliferation and evaluated the inhibitory effects of OSU-03012 on cell proliferation and migration. The data demonstrated that OSU-03012 dose-dependently inhibited A10 cell proliferation examined by Trypan blue, MTT and morphological alteration assays, and inhibited the levels of proliferation-related proteins, proliferating cell nuclear antigen (PCNA), phosphorylated ERK examined by western blotting. Additionally, 10 μM OSU-03012 also enhanced apoptosis examined using DAPI assay by regulating apoptosis-related proteins. Furthermore, compared with the control group, A10 cells treated with 10 μM OSU-03012 showed a lower ...Continue Reading
References
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L E ReidI M Kerr
Feb 1, 1988·Circulation·P W SerruysP G Hugenholtz
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Nov 1, 1995·The Journal of Clinical Investigation·R E BellasG E Sonenshein
Apr 1, 1995·Circulation·M A KarimA M Samarel
Jun 1, 1996·Nature Medicine·C IndolfiE V Avvedimento
Aug 10, 1996·Lancet·J M IsnerJ F Symes
May 26, 1998·The EMBO Journal·Z O JónssonU Hübscher
May 1, 1999·Circulation Research·C H SelzmanA Banerjee
May 18, 1999·Cardiovascular Research·M LabinazE R O'Brien
Dec 6, 2000·The Journal of Surgical Research·G JinS Chien
Feb 24, 2001·Cardiology in Review·C IndolfiM Chiariello
Jun 26, 2001·The Journal of Surgical Research·C H WuS Chien
Nov 13, 2001·Clinical and Experimental Pharmacology & Physiology·C L WainwrightR M Wadsworth
Feb 6, 2002·Cardiovascular Research·Michael Sturek, Hanumanth K Reddy
Nov 5, 2003·Journal of Plant Physiology·Edurne Gómez Roig, Jorge M Vázquez-Ramos
Jun 19, 2004·Cancer Research·Jiuxiang ZhuChing-Shih Chen
Apr 20, 2006·Molecular Pharmacology·Adly YacoubPaul Dent
Feb 3, 2007·Biochemical Pharmacology·Naoko Sakoguchi-OkadaToshiyuki Sasaguri
Aug 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shuhong ZhangSherif S Farag