Exploring the possibilities of capacitively coupled contactless conductivity detection in combination with liquid chromatography for the analysis of polar compounds using aminoglycosides as test case

Journal of Pharmaceutical and Biomedical Analysis
Péter JankovicsErwin Adams

Abstract

The analysis of highly polar (often charged) compounds which lack a strong UV absorbing chromophore is really challenging. Despite the numerous analytical methods published, the demand for a simple, robust and cheap technique for their analysis still persists. Here, reversed phase (RP) liquid chromatography (LC) with capacitively coupled contactless conductivity detection (C(4)D) was explored for the first time as a possible method for separation and detection of various aminoglycoside (AMG) antibiotics which were taken as typical test compounds: tobramycin (TOB), spectinomycin, streptomycin, amikacin, kanamycin A and kanamycin B. C(4)D was performed using a commercially available as well as a laboratory made cell. As ion-pairing reagents (IPR) four perfluorinated carboxylic acids were used: pentafluoropropionic acid, heptafluorobutyric acid, nonafluoropentanoic acid (NFPA) and pentadecafluorooctanoic acid (PDFOA). 0.125 mM NFPA-acetonitrile (ACN) (90:10) or 0.125 mM PDFOA-ACN (70:30) as mobile phases were suitable to detect TOB with reasonable retention times. However, NFPA was preferred for practical reasons. Its applicable concentration range in the mobile phase was strongly restricted by loss of chromatographic performance ...Continue Reading

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Citations

May 8, 2016·Talanta·Oliver Wahl, Ulrike Holzgrabe
Sep 9, 2015·Analytica Chimica Acta·Faten FaroukWilfried M A Niessen

Related Concepts

Acids
Spectinomycin
Amikacin
Antibiotics, Aminoglycoside
Liquid Chromatography
Diagnostic Techniques, Surgical
Ions
Kanamycin A
Laboratory
Laboratory Procedures

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