Exploring the potential of needle trap microextraction combined with chromatographic and statistical data to discriminate different types of cancer based on urinary volatomic biosignature

Analytica Chimica Acta
Priscilla Porto-FigueiraJosé S Câmara

Abstract

The worldwide high cancer incidence and mortality demands for more effective and specific diagnostic strategies. In this study, we evaluated the efficiency of an innovative methodology, Needle Trap Microextraction (NTME), combined with gas chromatography-mass spectrometry (GC-MS), for the establishment of the urinary volatomic biosignature from breast (BC), and colon (CC) cancer patients as well as healthy individuals (CTL). To achieve this, 40 mL of the headspace of acidified urine (4 mL, 20% NaCl, pH = 2), equilibrated at 50 °C during 40 min, were loaded through the DVB/Car1000/CarX sorbent inside the NTD, and subjected to a GC-MS analysis. This allowed the identification of 130 VOMs from different chemical families that were further processed using discriminant analysis through the partial least squares method (PLS-DA). Several pathways are over activated in cancer patients, being phenylalanine pathway in BC and limonene and pinene degradation pathway in CC the most relevant. Butanoate metabolism is also highly activated in both cancers, as well as tyrosine metabolism in a lesser extension. In BC the xenobiotics metabolism by cytochrome P450 and fatty acid biosynthesis are also differentially activated. Different clusters co...Continue Reading

Citations

Jul 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jorge A M PereiraJosé S Câmara
Apr 2, 2020·Journal of Veterinary Science·Marcela Valko-RokytovskáZuzana Kostecká
Aug 15, 2019·Scientific Reports·João Marcos Gonçalves BarbosaNelson Roberto Antoniosi Filho

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