Exploring the protein stability landscape: Bacillus subtilis lipase A as a model for detergent tolerance

Chembiochem : a European Journal of Chemical Biology
Alexander FultonKarl-Erich Jaeger

Abstract

A systematic study was conducted with Bacillus subtilis lipase A (BSLA) to determine the effect of every single amino acid substitution on detergent tolerance. BSLA is a minimal α/β-hydrolase of 181 amino acids with a known crystal structure. It can be expressed in Escherichia coli and is biochemically well characterized. Site saturation mutagenesis resulted in a library of 3439 variants, each with a single amino acid exchange as confirmed by DNA sequencing. The library was tested against four detergents, namely SDS, CTAB, Tween 80, and sulfobetaine. Surface remodeling emerged as an effective engineering strategy to increase tolerance towards detergents. Amino acid residues that significantly affect the tolerance for each of the four detergents were identified. In summary, this systematic analysis provides an experimental dataset to help derive novel protein engineering strategies as well as to direct modeling efforts.

References

Feb 1, 1984·Journal of Biochemistry·M SubramanianM P Venkatappa
Mar 1, 1993·Journal of Biotechnology·C von der OstenJ M Mikkelsen
Apr 20, 1999·Nature Biotechnology·J R CherryA H Pedersen
Nov 25, 2000·Biochimica Et Biophysica Acta·M le MaireJ V Moller
Aug 9, 2001·Journal of Molecular Biology·G van PouderoyenB W Dijkstra
Sep 1, 1955·Biochimica Et Biophysica Acta·A V FEWH C PARREIRA
Sep 24, 2004·Journal of Biotechnology·Vincent G H EijsinkGert Vriend
Nov 3, 2004·Biochimica Et Biophysica Acta·Annela M SeddonPaula J Booth
Apr 20, 2005·Journal of Colloid and Interface Science·Swati DeSusmita Das
May 8, 2007·Journal of Molecular Biology·Nobuhiko TokurikiDan S Tawfik
Apr 22, 2008·Protein Engineering, Design & Selection : PEDS·V BrissosK-E Jaeger
Jun 21, 2008·Chembiochem : a European Journal of Chemical Biology·Manfred T ReetzRenate Lohmer
Jan 3, 2009·Colloids and Surfaces. B, Biointerfaces·Nuzhat Gull Kabir-ud-Din
Jun 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jesse D Bloom, Frances H Arnold
Jun 30, 2009·Protein Engineering, Design & Selection : PEDS·Vladimir PotapovGideon Schreiber
Mar 17, 2010·Human Mutation·Sofia Khan, Mauno Vihinen
Jul 2, 2010·Chembiochem : a European Journal of Chemical Biology·Robert KouristUwe T Bornscheuer
Aug 4, 2010·Chembiochem : a European Journal of Chemical Biology·Helge Jochens, Uwe T Bornscheuer
Apr 19, 2011·Current Opinion in Chemical Biology·Thomas J MaglieryBrandon J Sullivan
Apr 21, 2011·Chembiochem : a European Journal of Chemical Biology·Helge JochensUwe T Bornscheuer
Sep 20, 2011·Journal of Molecular Biology·Md Zahid KamalNalam Madhusudhana Rao
May 12, 2012·Nature·U T BornscheuerK Robins
Oct 30, 2012·Journal of Chemical Information and Modeling·Niels J Christensen, Kasper P Kepp
Jan 15, 2013·Journal of Biotechnology·Nagihan AkbulutFüsun Gümüşel
Jul 19, 2013·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Yuval NovKarl-Erich Jaeger
Jul 19, 2013·Molecular BioSystems·Govindan RaghunathanSun-Gu Lee

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Citations

Jul 22, 2016·Chembiochem : a European Journal of Chemical Biology·Jan-Ytzen van der MeerGerrit J Poelarends
Feb 7, 2017·Canadian Journal of Microbiology·L RamnathR Govinden
Jan 21, 2017·The Journal of Physical Chemistry. B·Daniel KüblerKerstin Hoffmann-Jacobsen
Mar 24, 2016·PLoS Computational Biology·Prakash Chandra RathiHolger Gohlke
Dec 31, 2019·Chembiochem : a European Journal of Chemical Biology·Chao GuoGerrit J Poelarends
Nov 14, 2020·Chemistry : a European Journal·Haiyang CuiUlrich Schwaneberg
Dec 29, 2020·International Journal of Biological Macromolecules·Karen Rodríguez-NúñezRonny Martínez
Feb 9, 2021·Computational and Structural Biotechnology Journal·Francisca ContrerasUlrich Schwaneberg
Aug 14, 2020·Chembiochem : a European Journal of Chemical Biology·Chao GuoGerrit J Poelarends
Jul 2, 2021·Biotechnology and Bioengineering·Subrata PramanikMehdi D Davari
Oct 3, 2019·Bioconjugate Chemistry·Zhi ZouUlrich Schwaneberg
Jan 7, 2020·Journal of Chemical Information and Modeling·Christina NutschelHolger Gohlke
Aug 26, 2021·Computational and Structural Biotechnology Journal·Till El HarrarHolger Gohlke

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