Exploring the sequence diversity in glycoside hydrolase family 13_18 reveals a novel glucosylglycerol phosphorylase

Applied Microbiology and Biotechnology
Jorick FranceusTom Desmet

Abstract

In the carbohydrate-active enzyme database, GH13_18 is a family of retaining glycoside phosphorylases that act on α-glucosides. In this work, we explored the functional diversity of this family by comparing distinctive sequence motifs in different branches of its phylogenetic tree. A glycoside phosphorylase from Marinobacter adhaerens HP15 that was predicted to have a novel function was expressed and characterised. The enzyme was found to catalyse the reversible phosphorolysis of 2-O-α-D-glucosylglycerol with retention of the anomeric configuration, a specificity that has never been described before. Homology modelling, docking and mutagenesis were performed to pinpoint particular acceptor site residues (Tyr194, Ala333, Gln336) involved in the binding of glycerol. The new enzyme specificity provides additional insights into bacterial metabolic routes, being the first report of a phosphorolytic route for glucosylglycerol in a glucosylglycerol-producing organism. Furthermore, glucosylglycerol phosphorylase might be an attractive biocatalyst for the production of the osmolyte glucosylglycerol, which is currently produced on industrial scale by exploiting a side activity of the closely related sucrose phosphorylase. Family GH13_18 ...Continue Reading

References

Jan 14, 1970·Biochimica Et Biophysica Acta·E Belocopitow, L R Maréchal
Oct 31, 1998·Applied and Environmental Microbiology·K SaitoS Horinouchi
Oct 31, 2000·Bioscience, Biotechnology, and Biochemistry·F Takenaka, H Uchiyama
Apr 15, 2003·Plant Physiology·Ali FerjaniNorio Murata
Jun 14, 2003·Reproductive Biomedicine Online·J E Wagner
Mar 23, 2004·Analytical Biochemistry·Jeffrey D Gawronski, David R Benson
Jan 22, 2005·Applied Microbiology and Biotechnology·Sharon J Reid, Valerie R Abratt
Sep 8, 2006·Applied Microbiology and Biotechnology·Georg Lentzen, Thomas Schwarz
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Dec 1, 2009·Biotechnology Journal·Thornthan SawangwanBernd Nidetzky
Feb 22, 2011·International Journal of Systematic and Evolutionary Microbiology·Eva C KaeppelMatthias S Ullrich
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Aug 14, 2012·Chemistry : a European Journal·Tom DesmetAlexander Schiller
Nov 26, 2013·Nucleic Acids Research·Vincent LombardBernard Henrissat
Aug 22, 2015·Applied Microbiology and Biotechnology·Motomitsu Kitaoka
Nov 6, 2015·Journal of Agricultural and Food Chemistry·Karel De WinterTom Desmet
Dec 28, 2016·Glycobiology·Daniela Nunes-CostaNuno Empadinhas
Jul 30, 2017·Applied and Environmental Microbiology·Jorick FranceusTom Desmet

❮ Previous
Next ❯

Citations

Mar 27, 2019·Microbial Genomics·Alexandra S TauzinMickael Lafond
Aug 14, 2019·International Journal of Molecular Sciences·Jorick FranceusAndy-Mark W H Thunnissen
Apr 9, 2020·International Journal of Molecular Sciences·Jorick Franceus, Tom Desmet
Apr 9, 2021·Microbial Cell Factories·Katharina N SchwaigerBernd Nidetzky
May 16, 2021·Chembiochem : a European Journal of Chemical Biology·Jorick FranceusTom Desmet
Dec 12, 2020·Journal of Agricultural and Food Chemistry·Tong ZhangYuanxia Sun
Sep 21, 2021·Chembiochem : a European Journal of Chemical Biology·Marc De DonckerTom Desmet

❮ Previous
Next ❯

Datasets Mentioned

BETA
ADP98617

Methods Mentioned

BETA
PCR
electrophoresis
transglucosylation
light scattering
NMR

Software Mentioned

AutoDock VINA
Bitop
ProtParam
BLAST
PhyML
ClustalO
SigmaPlot
Schrödinger
YASARA
PyMOL

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.