Exploring the Spatial-Temporal Microbiota of Compound Stomachs in a Pre-weaned Goat Model

Frontiers in Microbiology
Yu LeiXiaolong Wang

Abstract

Ruminant animals possess a characteristic four-compartment stomach (rumen, reticulum, omasum, and abomasum) that is specialized for pre-intestinal digestion of plant materials. Of these four compartments, the rumen is the largest. The rumen's diverse microbial community has been well studied. However, the current understanding of microbial profiles in the reticulum, omasum and abomasum are lacking. In the present study, fluid samples from the reticulum, omasum, and abomasum of goats at 3, 7, 14, 21, 28, 42, and 56 days after birth, as well as the negative controls (NC) used for microbial DNA extraction, were subjected to 16S rRNA sequencing. By filtering operational taxonomic units (OTUs) in NC, distinct temporal distributions of microbes were observed in the different compartments, we showed that the OTUs in control samples had a large effect to the samples with low microbial density. In addition, Proteobacteria gradually decreased with age from days 3 to 56 in all three compartments, and the relative abundance of Bacteroidetes increased from 24.15% (Day 3) to 52.03% (Day 56) in abomasum. Network analysis revealed that Prevotellaceae_UGG-03 and Rikenellaceae_RC9 were positively correlated with Prevotella_1, lending support to ...Continue Reading

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Citations

May 6, 2019·FEMS Microbiology Reviews·Sarah Moraïs, Itzhak Mizrahi
Jul 31, 2019·Asian-Australasian Journal of Animal Sciences·Lovelia L MamuadSang Suk Lee
Jun 6, 2020·Scientific Reports·Clara Yieh Lin ChongJustin M O'Sullivan
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Dec 25, 2019·Scientific Reports·Lei WangGuofang Wu
Mar 6, 2021·Frontiers in Cellular and Infection Microbiology·Hailong DongQingxia Wu
Jun 25, 2021·Frontiers in Microbiology·Wenwen LiuHuayi Suo

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Datasets Mentioned

BETA
SRP090491

Methods Mentioned

BETA
PCR

Software Mentioned

R “ gplots package
bio I - Sanger Cloud Platform
RDP Classifier
UPARSE
PLS DA Discriminant
PLS
SPSS
FGR
R package “ decontam ”
PICRUSt

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