Exploring the Spatiotemporal Organization of Membrane Proteins in Living Plant Cells

Annual Review of Plant Biology
Li WangJinxing Lin

Abstract

Plasma membrane proteins have important roles in transport and signal transduction. Deciphering the spatiotemporal organization of these proteins provides crucial information for elucidating the links between the behaviors of different molecules. However, monitoring membrane proteins without disrupting their membrane environment remains difficult. Over the past decade, many studies have developed single-molecule techniques, opening avenues for probing the stoichiometry and interactions of membrane proteins in their native environment by providing nanometer-scale spatial information and nanosecond-scale temporal information. In this review, we assess recent progress in the development of labeling and imaging technology for membrane protein analysis. We focus in particular on several single-molecule techniques for quantifying the dynamics and assembly of membrane proteins. Finally, we provide examples of how these new techniques are advancing our understanding of the complex biological functions of membrane proteins.

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Citations

Feb 23, 2020·The New Phytologist·Haiyan ZhangJinxing Lin
Jul 19, 2018·Frontiers in Plant Science·Yue Niu, Yun Xiang
Aug 20, 2019·Cellular and Molecular Life Sciences : CMLS·Meng YuJinxing Lin
Aug 7, 2019·International Journal of Molecular Sciences·Li LiXiaoyi Shan
Jun 10, 2020·Nature Plants·Qi WangZhaosheng Kong
Jan 23, 2021·Trends in Plant Science·Jingjing XingJinxing Lin
Dec 15, 2020·Trends in Plant Science·Jingjing XingJinxing Lin
Jun 28, 2019·Journal of Agricultural and Food Chemistry·Chunhua Pang, Yan Gong
Sep 30, 2021·The Plant Cell·Leia ColinYvon Jaillais

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Methods Mentioned

BETA
transgenic
energy
fluorescence
super-resolution microscopy
optical microscopy
confocal microscopy
fluorescence correlation spectroscopy
FRET
FCS
fluorescence microscopy

Software Mentioned

SiMPull
smPPI
smSubunit
PALM
PCH
smFRET
STORM
FIDA
SpIDA
TIR

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