Exploring the yeast acetylome using functional genomics.

Cell
Supipi Kaluarachchi DuffyB J Andrews

Abstract

Lysine acetylation is a dynamic posttranslational modification with a well-defined role in regulating histones. The impact of acetylation on other cellular functions remains relatively uncharacterized. We explored the budding yeast acetylome with a functional genomics approach, assessing the effects of gene overexpression in the absence of lysine deacetylases (KDACs). We generated a network of 463 synthetic dosage lethal (SDL) interactions involving class I and II KDACs, revealing many cellular pathways regulated by different KDACs. A biochemical survey of genes interacting with the KDAC RPD3 identified 72 proteins acetylated in vivo. In-depth analysis of one of these proteins, Swi4, revealed a role for acetylation in G1-specific gene expression. Acetylation of Swi4 regulates interaction with its partner Swi6, both components of the SBF transcription factor. This study expands our view of the yeast acetylome, demonstrates the utility of functional genomic screens for exploring enzymatic pathways, and provides functional information that can be mined for future studies.

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B J Andrews, L A Moore
Apr 1, 1986·Journal of Bacteriology·J NikawaS Yamashita
Jul 1, 1994·Molecular and Cellular Biology·F R CrossA H Tinkelenberg
Jun 28, 1996·The Journal of Biological Chemistry·A A CarmenM Grunstein
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S E RundlettM Grunstein
May 14, 1999·Current Opinion in Genetics & Development·S Y Archer, R A Hodin
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·A A CarmenM Grunstein
Feb 17, 2000·The EMBO Journal·M A Martínez-BalbásT Kouzarides
Mar 16, 2000·The EMBO Journal·T Kouzarides
Feb 7, 2001·The Journal of Biological Chemistry·G MarzioM Giacca
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·B E BernsteinS L Schreiber
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·D Shore
Apr 3, 2001·Frontiers in Bioscience : a Journal and Virtual Library·C WangR G Pestell
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·J WuM Grunstein
Feb 9, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Reiner A Veitia
Jun 21, 2002·Methods in Enzymology·Vivien Measday, Philip Hieter
Jun 25, 2002·Methods in Enzymology·Jeffrey S SmithJef D Boeke
Nov 16, 2002·Science·Amy WangMichael Grunstein
Apr 3, 2003·Nature Reviews. Molecular Cell Biology·Siavash K Kurdistani, Michael Grunstein
Jul 11, 2003·Nature·Balázs PappLaurence D Hurst
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Jan 24, 2004·The EMBO Journal·Minkyu KimStephen Buratowski
Feb 7, 2004·Science·Amy Hin Yan TongCharles Boone
Mar 31, 2004·Journal of Molecular Biology·Ivan V GregorettiHolly V Goodson
Jun 24, 2004·Cell·Jonathan B Schaefer, Linda L Breeden
Oct 21, 2004·Molecular Cell·François RobertRichard A Young
Dec 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yufang ShaoXuejun Jiang
Dec 21, 2004·Nucleic Acids Research·Kevin P O'BrienErik L L Sonnhammer
May 7, 2005·Nature Biotechnology·Ryan Kelley, Trey Ideker
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Vivien MeasdayBrenda Andrews
Oct 26, 2005·Cell Death and Differentiation·T Yorimitsu, D J Klionsky
Nov 18, 2005·Gene·Michele A GlozakEdward Seto

❮ Previous
Next ❯

Citations

Apr 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Leslie MitchellKristin Baetz
Oct 2, 2013·Molecular and Cellular Biology·Man ZhangAles Vancura
Sep 30, 2014·FEBS Letters·Amornrat Naranuntarat JensenLaran T Jensen
Oct 30, 2015·Briefings in Functional Genomics·Michael Downey, Kristin Baetz
Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Mingtao HuangJens Nielsen
Jun 6, 2015·Cell·Yolanda T ChongBrenda J Andrews
Aug 27, 2013·Current Biology : CB·Michael DowneyDavid P Toczyski
Aug 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Supipi DuffyPhilip Hieter
Nov 9, 2014·Molecular & Cellular Proteomics : MCP·Michael DowneyDavid P Toczyski
Nov 3, 2016·Molecular & Cellular Proteomics : MCP·Matthew P TorresNiveda Sundararaman
Apr 26, 2017·Journal of Bacteriology·Valerie J Carabetta, Ileana M Cristea
Oct 19, 2014·Eukaryotic Cell·Luciano GaldieriAles Vancura
May 28, 2015·Antimicrobial Agents and Chemotherapy·Nilmar Silvio MorettiSergio Schenkman
May 17, 2014·Genetics·Ana Lilia Torres-Machorro, Lorraine Pillus
Oct 11, 2012·G3 : Genes - Genomes - Genetics·Alison C DouglasBrenda J Andrews
Oct 3, 2018·Frontiers in Plant Science·Barbara WójcikowskaMałgorzata D Gaj
Jul 24, 2013·The Journal of Biological Chemistry·Krista L Stilger, William J Sullivan
Jul 9, 2020·Data in Brief·Dimitris KyriakouAntonis Kirmizis
Dec 19, 2013·Journal of Cell Science·Philipp WildIvan Dikic
Apr 3, 2016·Cold Spring Harbor Protocols·Elena KuzminCharles Boone
Apr 3, 2016·Cold Spring Harbor Protocols·Erin B StylesBrenda J Andrews

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.