Exponential-phase expression of spvA of the Salmonella typhimurium virulence plasmid: induction in intracellular salts medium and intracellularly in mice and cultured mammalian cells.

Microbiology
Julie A WilsonP A Gulig

Abstract

The spv genes of Salmonella typhimurium and other non-typhoidal Salmonella serovars are essential for efficient systemic infection beyond the intestines in orally inoculated mice as a model for enteric fever. These virulence genes are not significantly expressed by salmonellae during exponential growth in L broth but are induced when the bacteria enter the stationary phase of growth. Using RNase protection analysis to directly measure spvA mRNA from the virulence plasmid of S. typhimurium, we found that spvA was maximally induced in an SpvR- and RpoS-dependent manner during exponential growth in intracellular Salts Medium, which mimics the intracellular environment of mammalian cells. A cloned spvA-lacZ operon fusion in S. typhimurium was induced intracellularly in periotoneal cells of mice, correlating in vivo intracellular gene expression with intracellular function of the spv genes in infected mice. spvA was also induced intracellularly in vitro within both Henle-407 intestinal epithelial cells and J774.A1 macrophage-like cells when the bacteria were replicating with exponential kinetics. Prevention of invasion of salmonellae with cytochalasin D inhibited spvA induction within tissue culture cells, indicating that salmonella...Continue Reading

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Citations

Jan 5, 2002·Microbes and Infection·F García-del Portillo
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D G MarshallC J Dorman
Apr 16, 2004·Clinical Microbiology Reviews·Cheng-Hsun ChiuChishih Chu
Jul 25, 2006·Infection and Immunity·Christopher D HerrenSuman Mukhopadhyay
Sep 14, 2002·Infection and Immunity·Holger BassoCarlos A Guzmán
Jan 24, 2006·Infection and Immunity·Sofia Eriksson YgbergMikael Rhen
Jan 7, 2009·Journal of Bacteriology·Javier F Mariscotti, Francisco García-del Portillo
May 3, 2008·Microbiology·Javier F Mariscotti, Francisco García-Del Portillo
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K E Unsworth, D W Holden
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H Smith
Aug 26, 2018·Genetics·Ioannis PassarisAbram Aertsen
Apr 16, 2005·Journal of Food Protection·Pamela A OlahCatherine M Logue
Feb 11, 1999·Journal of Bacteriology·B M AhmerF Heffron
Dec 1, 2011·EcoSal Plus·Regine Hengge

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