Exponential scaling of single-cell RNA-seq in the past decade

Nature Protocols
Valentine SvenssonSarah A Teichmann

Abstract

Measurement of the transcriptomes of single cells has been feasible for only a few years, but it has become an extremely popular assay. While many types of analysis can be carried out and various questions can be answered by single-cell RNA-seq, a central focus is the ability to survey the diversity of cell types in a sample. Unbiased and reproducible cataloging of gene expression patterns in distinct cell types requires large numbers of cells. Technological developments and protocol improvements have fueled consistent and exponential increases in the number of cells that can be studied in single-cell RNA-seq analyses. In this Perspective, we highlight the key technological developments that have enabled this growth in the data obtained from single-cell RNA-seq experiments.

References

Aug 1, 1992·Neuron·B LambolezJ Rossier
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J EberwineP Coleman
Oct 1, 1994·Analytical Biochemistry·H Z ShengP G Nelson
Feb 27, 2001·Nucleic Acids Research·L R BaughC P Hunter
Mar 27, 2001·Nature Reviews. Genetics·S H Orkin
Mar 30, 2002·Nature Biotechnology·Christoph A KleinGert Riethmüller
Apr 30, 2003·Neuron·Ian TietjenCatherine Dulac
Jun 24, 2004·Notes and Records of the Royal Society of London·Howard Gest
Oct 7, 2004·Genome Research·António PeixotoHenrique Veiga-Fernandes
Dec 7, 2007·Seminars in Immunology·Ivaylo I IvanovDan R Littman
Apr 8, 2009·Nature Methods·Fuchou TangM Azim Surani
Jan 13, 2010·Immunology and Cell Biology·Jinfang Zhu
Jul 24, 2012·Nature Biotechnology·Daniel RamsköldRickard Sandberg
Aug 23, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Scott BrouiletteKenta Yashiro
Sep 4, 2012·Cell Reports·Tamar HashimshonyItai Yanai
Apr 6, 2013·Nature Protocols·Linas MazutisJohn A Heyman
Jul 31, 2013·Nature Reviews. Genetics·Ehud ShapiroSten Linnarsson
Sep 24, 2013·Nature Methods·Simone PicelliRickard Sandberg
Sep 24, 2013·Nature Methods·Philip BrenneckeMarcus G Heisler
Mar 1, 2014·Science·Je Hyuk LeeGeorge M Church
May 23, 2015·Molecular Cell·Aleksandra A KolodziejczykSarah A Teichmann
Jun 7, 2015·Genome Biology·Sayantan BosePeter A Sims
Oct 3, 2015·Genome Research·Cole Trapnell
Aug 30, 2016·Nature Neuroscience·Jean-Francois PoulinRajeshwar Awatramani
Sep 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey R MoffittXiaowei Zhuang
Oct 28, 2016·Cell Systems·Mauro J MuraroAlexander van Oudenaarden
Oct 16, 2016·Nature Communications·Sanja VickovicJoakim Lundeberg
Nov 8, 2016·Nature Reviews. Genetics·Detlev ArendtGünter P Wagner
Dec 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey R MoffittXiaowei Zhuang
Dec 22, 2016·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Je H Lee

❮ Previous
Next ❯

Citations

Jun 26, 2018·PLoS Computational Biology·Luke ZappiaAlicia Oshlack
May 5, 2018·Annual Review of Genomics and Human Genetics·Lia ChappellThierry Voet
Feb 12, 2019·Nature Methods·George C LindermanYuval Kluger
Dec 15, 2018·The FEBS Journal·Akshitkumar M MistryJonathan M Irish
May 3, 2019·BMC Bioinformatics·Arnaud BonnaffouxOlivier Gandrillon
May 11, 2019·Bioinformatics·Páll MelstedLior Pachter
May 28, 2019·Immunology and Cell Biology·Valentine Svensson
Mar 9, 2019·Annual Review of Analytical Chemistry·Ian S GilmoreCornelius L Pieterse
Jun 22, 2019·Nucleic Acids Research·Jurrian K de KanterFrank C P Holstege
Aug 8, 2019·Mass Spectrometry Reviews·Irena DapicGarry L Corthals
Sep 11, 2019·Nature Methods·Hannah A PlinerCole Trapnell
Feb 27, 2018·Nature Methods·Charlotte Soneson, Mark D Robinson
Nov 26, 2019·Bioinformatics·Roman HilljeLucilla Luzi
Dec 22, 2019·Cells·Daniel OsorioJames J Cai
Feb 1, 2020·Foodborne Pathogens and Disease·Bin WangShaopeng Gu
Jan 3, 2020·Current Opinion in Oncology·Charlotte K Brierley, Adam J Mead
Feb 6, 2020·Journal of Bioinformatics and Computational Biology·Huiqing WangChunlin Dong
Mar 1, 2020·Tissue Engineering. Part C, Methods·Diwakar TuragaTodd C McDevitt
Dec 4, 2019·Bioinformatics·Saskia Freytag, Ryan Lister
Mar 17, 2020·Bioinformatics·Valentine SvenssonLior Pachter
May 1, 2019·Nature Communications·Hatice S Kaya-OkurSteven Henikoff
Feb 23, 2020·Annual Review of Immunology·Mirjana EfremovaKylie R James
Apr 28, 2020·PLoS Computational Biology·Yuanchao ZhangDeanne M Taylor
Mar 4, 2020·The Journal of Pathology·Benjamin J Stewart, Menna R Clatworthy
Jan 15, 2020·Cold Spring Harbor Perspectives in Biology·Iwo Kucinski, Berthold Gottgens
Aug 12, 2018·Genome Biology·Ángeles Arzalluz-Luque, Ana Conesa
Jul 13, 2018·Frontiers in Immunology·Daniel J KunzKylie R James
Mar 19, 2019·Frontiers in Genetics·Samantha L GoldmanChristopher E Mason
Jun 5, 2019·Genome Biology·Giovanni IaconoHolger Heyn
Jun 30, 2019·Development·Bo Xia, Itai Yanai
Dec 25, 2019·Nature Biotechnology·Jase GehringLior Pachter
Feb 9, 2020·Genome Biology·David LähnemannAlexander Schönhuth
Feb 12, 2020·Nature Reviews. Neuroscience·Xinzhu YuBaljit S Khakh
Apr 10, 2020·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Rizqah Kamies, Celia P Martinez-Jimenez
May 15, 2020·Molecular Psychiatry·Amanda J PriceDaniel R Weinberger
Jul 6, 2020·European Respiratory Review : an Official Journal of the European Respiratory Society·Michael J AlexanderPaul A Reyfman
Jun 11, 2020·Nature Biotechnology·Elisabetta MereuHolger Heyn

❮ Previous
Next ❯

Related Concepts

Related Feeds

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.

Related Papers

Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis
Andrew NicolaidesInternational Union of Angiology and Union Internationale du Phlebologie
Journal of Genetics and Genomics = Yi Chuan Xue Bao
Yikai WangTianwei Yu
Australian and New Zealand Journal of Public Health
Anne BuistBryanne Barnett
Journal of Pain & Palliative Care Pharmacotherapy
Elon Eisenberg
© 2022 Meta ULC. All rights reserved