PMID: 2496108May 1, 1989Paper

Export and intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae

Journal of Bacteriology
D W DorwardR C Judd

Abstract

Naturally elaborated membrane bleb material is frequently observed in cultures of Neisseria gonorrhoeae. This material was purified and analyzed for protein, lipopolysaccharide, and nucleic acid content. The electrophoretic protein profiles of two bleb-rich fractions, called BI and BII, were distinct, with only BII containing lipopolysaccharide and outer membrane proteins I and III. Both fractions contained RNA, circular DNA, and linear DNA. Exogenous pancreatic DNase I appeared to hydrolyze all bleb-associated DNA in fraction BI and the linear DNA in fraction BII. The circular DNA molecules associated with fraction BII resisted digestion. Electron microscopy of the bleb fractions verified their DNA content. Fixing blebs with glutaraldehyde before mounting them for microscopy prevented release of internal DNA. Such fixation produced little change in the micrographs of BI; however, only traces of DNA were observed in fixed BII preparations. Incubation of wild-type gonococci in mixtures of DNase and blebs purified from antibiotic-resistant strains resulted in efficient exchange of penicillinase-specifying R plasmids. Recipients incorporated plasmids independently of endogenous and exogenous chromosomal streptomycin resistance mar...Continue Reading

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Citations

Jan 1, 1994·Microbiology and Immunology·K AhmedK Matsumoto
Jan 1, 1993·Microbiology and Immunology·K AhmedK Matsumoto
Mar 3, 2011·FEMS Microbiology Ecology·Hiroshi Xavier ChiuraBranko Velimirov
Oct 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Johanna RiveraArturo Casadevall
Feb 26, 2016·Biotechnology Journal·Leo van der PolPeter van der Ley
Feb 10, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jakob ThannesbergerChristoph Steininger
Jun 18, 2017·International Journal of Molecular Sciences·Natalie J Bitto, Maria Kaparakis-Liaskos
May 16, 2017·The Journal of Antimicrobial Chemotherapy·Somdatta ChatterjeeSulagna Basu
Feb 21, 2018·FEMS Microbiology Letters·Anubrata Ghosal
Aug 8, 2018·Wiener medizinische Wochenschrift·Branko Velimirov, Carmen Ranftler
Apr 9, 2020·Frontiers in Microbiology·Julie C Caruana, Scott A Walper
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julia B PatroneDaniel C Stein
Nov 19, 2002·FEMS Microbiology Letters·Jun-ichiro HayashiHoward K Kuramitsu
Feb 13, 2009·Molecular Microbiology·Mikhail KudryashevFriedrich Frischknecht
Apr 19, 2008·Mass Spectrometry Reviews·Eun-Young LeeYong Song Gho
Dec 15, 2010·Seminars in Immunopathology·Can M UnalKristian Riesbeck
Nov 14, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Khalid Al-NedawiJohn Bienenstock
Mar 25, 2014·Applied and Environmental Microbiology·Shweta FulsundarKaare M Nielsen
Aug 11, 2015·Frontiers in Microbiology·Madhab K Chattopadhyay, Medicharla V Jaganandham
May 29, 2013·Extremophiles : Life Under Extreme Conditions·Marleen van WolferenSonja-Verena Albers
Sep 4, 2015·Current Genetics·Dina VorkapicStefan Schild
Jun 9, 2016·Journal of Bacteriology·Mark T AndersonH Steven Seifert
May 16, 2015·Nature Reviews. Immunology·Maria Kaparakis-Liaskos, Richard L Ferrero

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