Abstract
The objective of this study performed in Ancistrus multispinis was to evaluate whether sublethal doses of deltamethrin (DM) could affect lipopolysaccharide (LPS)-induced leukocyte migration to the peritoneal cavity and the production of nitric oxide (NO) by kidney macrophages exposed to LPS and/or DM. For cell migration studies, 10 fish were exposed to DM 0.1 or 0.3 mg kg(-1) or vehicle for 96 h. After this period, each group received LPS (0.1 microg kg(-1)) or saline and the cells were collected after 4h. For the production of NO, kidney macrophages were isolated from eight fish and the cells were incubated for 24 or 48 h with LPS (10 microg mL(-1)) and DM (0.3 and 1 microg mL(-1)). The results were analyzed using ANOVA followed by Bonferroni multiple comparisons test. A small leukocyte population (530+/-30.8 cells microL(-1)) was naturally observed in the peritoneal cavity, however after inoculation of LPS, the number of leukocytes was increased by 157%. For the fish treated with DM 0.1 and 0.3 mg kg(-1), total leukocytes had increased by 100 and 162%, respectively. The combination of LPS with the higher dose of DM showed a potentiating or additive effect. The production of NO was increased in all the treated groups in relati...Continue Reading
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