Exposure of Daphnia magna to trichloroethylene (TCE) and vinyl chloride (VC): evaluation of gene transcription, cellular activity, and life-history parameters

Ecotoxicology and Environmental Safety
Magali HoudeFrançois Cloutier

Abstract

Trichloroethylene (TCE) is a ubiquitous contaminant classified as a human carcinogen. Vinyl chloride (VC) is primarily used to manufacture polyvinyl chloride and can also be a degradation product of TCE. Very few data exist on the toxicity of TCE and VC in aquatic organisms particularly at environmentally relevant concentrations. The aim of this study was to evaluate the sub-lethal effects (10 day exposure; 0.1; 1; 10 µg/L) of TCE and VC in Daphnia magna at the gene, cellular, and life-history levels. Results indicated impacts of VC on the regulation of genes related to glutathione-S-transferase (GST), juvenile hormone esterase (JHE), and the vitelline outer layer membrane protein (VMO1). On the cellular level, exposure to 0.1, 1, and 10 µg/L of VC significantly increased the activity of JHE in D. magna and TCE increased the activity of chitinase (at 1 and 10 µg/L). Results for life-history parameters indicated a possible tendency of TCE to affect the number of molts at the individual level in D. magna (p=0.051). Measurement of VG-like proteins using the alkali-labile phosphates (ALP) assay did not show differences between TCE treated organisms and controls. However, semi-quantitative measurement using gradient gel electrophore...Continue Reading

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Citations

Sep 1, 2015·Aquatic Toxicology·Pål A OlsvikBjørn T Lunestad
Aug 16, 2015·Archives of Environmental Contamination and Toxicology·Jes J RasmussenPoul L Bjerg
Aug 4, 2016·Environmental Science & Technology·Shu ZhangShaily Mahendra
Oct 29, 2018·Environmental Monitoring and Assessment·Mina JoWoo-Keun Kim
Dec 22, 2019·Environmental Science and Pollution Research International·Feyza Icoglu Aksakal, Harun Arslan
Nov 15, 2018·Environmental Science and Pollution Research International·Zouhour Ouanes-Ben OthmenAmel Hamza-Chaffai

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