Exposure of Escherichia coli to acid habituation conditions sensitizes it to alkaline stress

Letters in Applied Microbiology
R J Rowbury, N H Hussain

Abstract

Escherichia coli transferred from pHo 7.0 to pHo 5.5 or 6.0 became alkali-sensitive by a rapidly induced phenotypic response. Alkali sensitization was reduced at pHo 5.0 and virtually abolished at pHo 6.5. The response was triggered by cytoplasmic rather than external or periplasmic acidification and de novo protein synthesis was needed. Alkali sensitivity failed to appear at pHo 5.5 plus DNA gyrase inhibitors and was markedly reduced by himA, himD, hns, ompC and nhaA lesions. A tonB deletion mutant showed alkali sensitivity at pHo 7.0. Alkali sensitivity induction was not subject to catabolite repression nor was it appreciably affected by a relA lesion. Acid-induced cells were more sensitive to alkali damage to both DNA and beta-galactosidase and to alkali inhibition of beta-galactosidase induction. Alkali sensitization induced at pHo 5.5 may involve NhaB loss.

References

Sep 1, 1992·Journal of General Microbiology·A D Thomas, I R Booth
Feb 1, 1990·Journal of Bacteriology·J W Foster, H K Hall
Feb 1, 1988·Canadian Journal of Microbiology·C F ReakesR J Rowbury
Aug 4, 1983·Nature·B Demple, J Halbrook
Jun 1, 1993·Applied and Environmental Microbiology·G J Leyer, E A Johnson
Jun 1, 1993·Journal of Molecular and Cellular Cardiology·J KählerR J Bing

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Citations

Mar 4, 2000·Applied and Environmental Microbiology·M SatoH Kobayashi
Feb 16, 2006·Letters in Applied Microbiology·M KaeriyamaH Kobayashi
Jun 1, 1996·Letters in Applied Microbiology·R J RowburyM Goodson
Oct 29, 2004·Science Progress·Hiromi Saito, Hiroshi Kobayashi

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