Exposure of Monocytes to Lipoarabinomannan Promotes Their Differentiation into Functionally and Phenotypically Immature Macrophages

Journal of Immunology Research
Leslie Chávez-GalánIsabel Sada-Ovalle

Abstract

Lipoarabinomannan (LAM) is a lipid virulence factor secreted by Mycobacterium tuberculosis (Mtb), the etiologic agent of tuberculosis. LAM can be measured in the urine or serum of tuberculosis patients (TB-patients). Circulating monocytes are the precursor cells of alveolar macrophages and might be exposed to LAM in patients with active TB. We speculated that exposing monocytes to LAM could produce phenotypically and functionally immature macrophages. To test our hypothesis, human monocytes were stimulated with LAM (24-120 hours) and various readouts were measured. The study showed that when monocytes were exposed to LAM, the frequency of CD68(+), CD33(+), and CD86(+) macrophages decreased, suggesting that monocyte differentiation into mature macrophages was affected. Regarding functionality markers, TLR2(+) and TLR4(+) macrophages also decreased, but the percentage of MMR(+) expression did not change. LAM-exposed monocytes generated macrophages that were less efficient in producing proinflammatory cytokines such as TNF-α and IFN-γ; however, their phagocytic capacity was not modified. Taken together, these data indicate that LAM exposure influenced monocyte differentiation and produced poorly functional macrophages with a diffe...Continue Reading

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Citations

Jun 30, 2019·Microbiology and Immunology·Leslie Chávez-GalánRicardo Lascurain
Dec 4, 2020·International Journal of Molecular Sciences·Julio Flores-GonzalezLeslie Chavez-Galan
May 4, 2021·Frontiers in Microbiology·Julio FloresLeslie Chavez-Galan
Jun 29, 2021·Journal of Endodontics·Chloé Le FournisImad About

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Methods Mentioned

BETA
sandwich immunoassay
flow cytometry
FACS
ELISA

Software Mentioned

GraphPad
FACS Diva

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