Exposure of RBL-2H3 mast cells to Ag(+) induces cell degranulation and mediator release

Biochemical and Biophysical Research Communications
Y SuzukiK Shimizu

Abstract

There is a growing need to understand the impact of environmental sulfhydryl group-reactive heavy metals on the immune system. Here we show that Ag(+) induces mast cell degranulation, as does the aggregation of the high affinity immunoglobulin E receptor (FcepsilonRI). Micromolar quantities of Ag(+) specifically induced degranulation of mast cell model rat basophilic leukemia (RBL-2H3) cells without showing cytotoxicity. The Ag(+)-mediated degranulation could be observed as rapidly as 5 min after the addition of the ions. Ag(+) also induced a rapid change in tyrosine phosphorylation of multiple cellular proteins including the focal adhesion kinase but not Syk kinase. The Syk-selective inhibitor piceatannol and the Src family-selective tyrosine kinase inhibitor PP1 dose-dependently inhibited FcepsilonRI-mediated degranulation, whereas neither compound inhibited the Ag(+)-mediated degranulation. Furthermore, likewise FcepsilonRI aggregation, Ag(+) also induced leukotriene secretion. These results show that Ag(+) activates RBL-2H3 mast cells through a tyrosine phosphorylation-linked mechanism, which is distinct from that involved in FcepsilonRI-mediated activation.

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Citations

Mar 13, 2010·Journal of Neuroinflammation·Duraisamy KempurajTheoharis C Theoharides
May 3, 2003·Environmental Health Perspectives·Aurelia Walczak-DrzewieckaJaroslaw Dastych
Jun 30, 2011·The Journal of Veterinary Medical Science·Pattwat ManeewattanapinyoTheerayuth Kaewamatawong
Mar 26, 2014·Pharmaceutical Research·Byeongtaek Oh, Chi H Lee
Aug 31, 2010·Journal of Biomedical Optics·Wenzhong YangDugyoung Kim
Sep 7, 2011·Phytochemistry·Megumi FurukawaYasuo Fujimoto
Nov 21, 2007·Toxicology Letters·X Chen, H J Schluesener
Jun 17, 2009·Macromolecular Bioscience·Vichayarat RattanaruengsrikulPitt Supaphol
May 29, 2010·Immunopharmacology and Immunotoxicology·Zeinab ShavandiKiumarz Nazari Moghaddam
Oct 9, 2019·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Christina S ThorntonJohn M Conly
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshihiro SuzukiChisei Ra
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eric GaudreaultMarek Rola-Pleszczynski
Apr 30, 2009·Journal of Leukocyte Biology·Toshio InoueChisei Ra

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