Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Journal of Materials Science. Materials in Medicine
Liangjiao ChenLongquan Shao

Abstract

Inflammatory effects are significant elements of the immune response to biomaterials. Previously, we reported inflammatory effects in response to dicalcium silicate (Ca2SiO4, C2S) particles. However, the immunological effects of C2S coatings have not been studied. C2S often used as coatings materials in orthopedic and dentistry applications. It may have different effect from C2S particles. Further, it remains unclear whether C2S coating is equally biocompatible as 45S5 coating. The aim of this study was to test the cytotoxicity and pro-inflammatory effects of C2S coating on RAW 264.7 macrophages. C2S and 45S5 coatings were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive analysis (EDS) and X-ray diffraction (XRD). inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to detect ionic concentrations after soaking coated discs in medium. The cytotoxicity of C2S and 45S5 coatings against RAW 264.7 macrophages was measured using the LDH Cytotoxicity Assay Kit, Cell Counting Kit-8 (CCK-8) assays and flow cytometry for apoptosis assays. The gene and protein expression of TNF-α, IL-6 and IL-1β were detected using RT-q PCR and ELISA, respectively. The tested c...Continue Reading

References

Jan 5, 2002·Biomaterials·Xuanyong LiuChuanxian Ding
Feb 9, 2002·Journal of Biomedical Materials Research·M BosettiM Cannas
Dec 23, 2003·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Julian R Jones, Larry L Hench
Apr 2, 2004·Clinical Orthopaedics and Related Research·Kazuo HirakawaTomoyuki Saito
Jul 1, 2004·Journal of Biomedical Materials Research. Part a·Ali K RefaiJ Douglas Waterfield
Sep 7, 2004·Journal of Materials Science. Materials in Medicine·T M LeeM R Yang
Mar 29, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Zhongru GouJunying Wang
Jul 13, 2005·Journal of Biomedical Materials Research. Part a·Xuanyong LiuPaul K Chu
Jul 10, 2007·Journal of Biomedical Materials Research. Part a·Adam M KaufmanArun S Shanbhag
Feb 24, 2009·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Junying SunFanhao Meng
Jul 27, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Maria Giovanna GandolfiCarlo Prati
Jan 18, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Borbála PazárNathalie Busso
May 15, 2012·Journal of Materials Science. Materials in Medicine·Ilaria CacciottiLaura Montanaro
Sep 4, 2012·Tissue & Cell·A Scislowska-CzarneckaE Kolaczkowska
Oct 20, 2012·Clinical Oral Implants Research·Bart A J A van OirschotJohn A Jansen
Jun 28, 2013·International Journal of Molecular Sciences·Sharmy Saimon ManoAkiyoshi Taniguchi
Jul 5, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Piedad N de AzaJose L Calvo-Guirado
Aug 6, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Piedad N De AzaLuis Meseguer-Olmo
Jan 18, 2014·Materials Science & Engineering. C, Materials for Biological Applications·G M NelsonA G McDonald
Jan 30, 2014·Tissue Engineering. Part a·Simon D NewmanMolly M Stevens
Jun 4, 2014·Mediators of Inflammation·Stefan LandgraeberNadim James Hallab
Jan 17, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Chen LiangjiaoShao Longquan
Jun 21, 2015·Acta Biomaterialia·Raluca IonPatrik Schmuki

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Citations

Mar 3, 2017·Journal of Materials Science. Materials in Medicine·Wei ZhuXisheng Weng
Dec 12, 2017·International Endodontic Journal·L M S Castro-RaucciP T de Oliveira
Aug 22, 2021·Acta Biomaterialia·Kai ZhengAldo R Boccaccini

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