PMID: 3763824Jan 1, 1986Paper

Exposure of tryptophanyl residues in alpha-lactalbumin and lysozyme. Quantitative determination by fluorescence quenching studies

Radiation and Environmental Biophysics
A M Edwards, E Silva

Abstract

The effect of iodide ion on the tryptophyl fluorescence of the homologous proteins lysozyme and alpha-lactalbumin in their native form, as well as in their modified structures and in fragments from these proteins was studied. By assessing the contribution to the total fluorescence of the exposed and buried Trp residues, and of the respective fluorescence quantum yields, the quantification of the number of Trp exposed to the solvent for all the species studied was possible. Both native proteins show an important increase in the number of Trp residues exposed to the solvent when treated with denaturing agents. The peptides L-II (aa 13-105) and alpha-I (aa 1-90) from lysozyme and alpha-lactalbumin, respectively, showed Trp residues with different degree of exposure, whereas the smaller fragments, L-III (aa 106-129) and alpha-II (aa 91-123), had all their Trp residues exposed to the solvent.

References

May 14, 1975·Journal of the American Chemical Society·D V Bent, E Hayon
Jan 1, 1985·Radiation and Environmental Biophysics·I Ferrer, E Silva
Jan 1, 1985·Radiation and Environmental Biophysics·A M Edwards, E Silva
Apr 18, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C C BlakeV R Sarma
Oct 1, 1973·Photochemistry and Photobiology·E A BursteinM N Ivkova
Jun 10, 1974·Radiation and Environmental Biophysics·E SilvaK Dose
Jul 13, 1966·Biochimica Et Biophysica Acta·J E Churchich
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Jul 1, 1981·Analytical Biochemistry·M R Eftink, C A Ghiron
Jan 1, 1981·Radiation and Environmental Biophysics·I Ferrer, E Silva

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Citations

Nov 16, 1992·Chemico-biological Interactions·J Gonzalez-JimenezI Cayre
Apr 30, 1992·Journal of Photochemistry and Photobiology. B, Biology·R UgarteE Silva
Oct 31, 2001·Journal of Photochemistry and Photobiology. B, Biology·A M Edwards, E Silva
Jan 1, 1988·Radiation and Environmental Biophysics·D L VanderMeulen, M M Judy
Jan 1, 1991·Radiation and Environmental Biophysics·G Tapia, E Silva
Aug 12, 2019·Colloids and Surfaces. B, Biointerfaces·Mbaye NdourSebastien Balme
Feb 1, 1989·International Journal of Biological Macromolecules·C C Yang, L S Chang

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