Exposure to 60% oxygen promotes migration and upregulates angiogenesis factor secretion in breast cancer cells

Medical Gas Research
P D CrowleyH C Gallagher

Abstract

Peri-operative factors, including anaesthetic drugs and techniques, may affect cancer cell biology and clinical recurrence. In breast cancer cells, we demonstrated that sevoflurane promotes migration and angiogenesis in high fractional oxygen but not in air. Follow-up analysis of the peri-operative oxygen fraction trial found an association between high inspired oxygen during cancer surgery and reduced tumor-free survival. Here we evaluated effects of acute, high oxygen exposure on breast cancer cell viability, migration and secretion of angiogenesis factorsin vitro. MDA-MB-231 and MCF-7 breast cancer cells were exposed to 21%, 30%, 60%, or 80% v/v O2for 3 hours. Cell viability at 24 hours was determined by MTT and migration at 24 hours with the Oris™ Cell Migration Assay. Secretion of angiogenesis factors at 24 hours was measuredviamembrane-based immunoarray. Exposure to 30%, 60% or 80% oxygen did not affect cell viability. Migration of MDA-MB-231 and MCF-7 cells was increased by 60% oxygen (P= 0.012 andP= 0.007, respectively) while 30% oxygen increased migration in MCF-7 cells (P= 0.011). These effects were reversed by dimethyloxaloylglycine. In MDA-MB-231 cells high fractional oxygen increased secretion of angiogenesis facto...Continue Reading

References

Nov 16, 2013·British Journal of Anaesthesia·D S Martin, M P W Grocott

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Citations

Aug 30, 2019·Current Opinion in Anaesthesiology·Lothar A SchwarteStephan A Loer

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Methods Mentioned

BETA
Assay

Software Mentioned

Graphpad
GelEval
Prism

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