PMID: 9444962Jan 28, 1998Paper

Exposure to chronic hypoxia induces qualitative changes of collagen in the walls of peripheral pulmonary arteries

Life Sciences
J Novotná, J Herget

Abstract

Qualitative changes of vascular wall matrix collagens in chronic hypoxic pulmonary hypertension were studied by gel electrophoresis. Male adult rats (n = 12) were exposed to hypoxia (FiO2 = 0.1, 3 wks). Control rats (n = 13) were kept in air. Samples of peripheral pulmonary arteries (PPA, diam. 100-400 microm), main branches of pulmonary artery, and aorta were dissected. Arterial samples were treated with 4M guanidine-HCl to remove noncollagenous moieties and the collagenous stroma was dissolved by limited pepsin digestion at low pH. Low molecular mass peptides (M. W. approx. 76 and 66 kD) were detected in the gel electrophoretic profile of collagen peptides of PPA of the chronically hypoxic animals and in aorta of both hypoxic and normoxic groups. These peptides were absent in the PPA of normoxic rats. Since the 76 kD peptide bound anticollagen type I antibodies, it appears to be of collagenous nature and it may be the result of collagenolytic activity in PPA isolated from hypoxic lungs. This was confirmed by zymography. We conclude that exposure of rats to chronic hypoxia results in the presence of low molecular mass peptides in the wall matrix of PPA which resemble those found in aorta of normoxic animals. Collagenolytic act...Continue Reading

References

Dec 4, 1992·Annals of the New York Academy of Sciences·G MurphyJ J Reynolds
Sep 28, 1990·Biochemical and Biophysical Research Communications·H SaariT Sorsa
Apr 1, 1990·Circulation Research·G J PoianiD J Riley
Nov 1, 1989·The American Review of Respiratory Disease·W C ParksK R Stenmark
Apr 14, 1988·Biochimica Et Biophysica Acta·J C MonboisseC Ferradini
Jan 1, 1985·Annals of the New York Academy of Sciences·J EngelR Timpl
Apr 1, 1986·Chest
Oct 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·J Gross, Y Nagai
Jan 1, 1980·Annual Review of Biochemistry·P Bornstein, H Sage
Dec 1, 1984·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·J S KerrH M Frankel
Jun 1, 1995·American Journal of Respiratory and Critical Care Medicine·U SpecksR Timpl
May 1, 1994·American Journal of Respiratory and Critical Care Medicine·C A TozziD J Riley
Mar 1, 1994·Fetal Diagnosis and Therapy·L MarpeauJ Milliez
Nov 1, 1993·Archives of Disease in Childhood·J HendersonD Yeates
Dec 1, 1995·The European Respiratory Journal·N F Voelkel, R M Tuder
Dec 15, 1995·The Journal of Physiology·K NakanishiH Ohno
Jun 1, 1996·Blood Reviews·D A LaneS L Thein
Nov 8, 1996·The Journal of Biological Chemistry·S M KraneY Eeckhout
Feb 1, 1997·Human Molecular Genetics·N G LarssonD A Clayton

❮ Previous
Next ❯

Citations

Oct 3, 2002·The Journal of Emergency Medicine·Dan S MoselyCurtis J Hunter
Feb 22, 2007·AJR. American Journal of Roentgenology·Silke GrabherrRichard Dirnhofer
Apr 5, 2007·Cell Biochemistry and Biophysics·Juan M EsteveLuc Maroteaux
Apr 1, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jan HergetVaclav Hampl
Dec 23, 2011·Physiological Research·H MaxováM Vízek
Oct 4, 2000·Physiological Reviews·V Hampl, J Herget
Aug 20, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Pierre BonnetVéronique Eder
Apr 1, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·Z Deyl, I Miksík
Apr 22, 2003·Progress in Cardiovascular Diseases·Xing Li WangJian Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.