Exposure to Gestational Diabetes Enriches Immune-Related Pathways in the Transcriptome and Methylome of Human Amniocytes.

The Journal of Clinical Endocrinology and Metabolism
Sara E PinneyPaul Zhipang Wang

Abstract

Gestational diabetes (GDM) has profound effects on the intrauterine metabolic milieu and is linked to obesity and diabetes in offspring, but the mechanisms driving these effects remain largely unknown. Alterations in DNA methylation and gene expression in amniocytes exposed to GDM in utero represent a potential mechanism leading to metabolic dysfunction later in life. To profile changes in genome-wide DNA methylation and expression in human amniocytes exposed to GDM. A nested case-control study (n = 14 pairs) was performed in amniocytes matched for offspring sex, maternal race/ethnicity, maternal age, gestational age at amniocentesis, and gestational age at birth. Sex-specific genome-wide DNA methylation analysis and RNA-sequencing were completed and differentially methylated regions (DMRs) and gene expression changes were identified. Ingenuity pathway analysis identified biologically relevant pathways enriched after GDM exposure. In silico high-throughput chromosome conformation capture (Hi-C) analysis identified potential chromatin interactions with DMRs. Expression of interferon-stimulated genes was increased in GDM amniocytes, accounting for 6 of the top 10 altered genes (q < 0.05). Enriched biological pathways in GDM amnio...Continue Reading

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Citations

Jul 25, 2021·International Journal of Molecular Sciences·Dominik Franciszek DłuskiMaciej Skrzypczak
Sep 4, 2021·The Journal of Clinical Endocrinology and Metabolism·Xinmei HuangJun Liu

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