Exposure to modeled microgravity induces metabolic idleness in malignant human MCF-7 and normal murine VSMC cells

FEBS Letters
Rita CoinuGiuseppe Palumbo

Abstract

We investigated the effect of modeled microgravity (MMG) on normal vascular smooth muscle cells (VSMC) and neoplastic human breast cancer cells (MCF-7). In both cell types, MMG induced partial arrest in G2M and increased p14-3-3, HSP70, HSP60 and p21 expression. Cells synchronized by 24h starvation reentered the normal cycle within 24h if released in complete medium and exposed to normal gravity, but not if exposed to MMG. Similarly, MMG prevented VSMC and MCF-7 cells from overcoming growth arrest and re-synthesizing DNA. This study shows that cells adjust their metabolic rate in response to MMG.

References

Jan 1, 1995·The Journal of Clinical Investigation·G Minowada, W J Welch
Apr 10, 1996·Experimental Cell Research·M Hughes-Fulford, M L Lewis
Aug 26, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M L LewisE H Piepmeier
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J VassyD Schoevaert
Oct 24, 2003·Biochimica Et Biophysica Acta·Sofia I M CarlssonJeanette A M Maier

❮ Previous
Next ❯

Citations

Oct 26, 2012·Cell Biochemistry and Biophysics·Hongyan KangXiaoyan Deng
Jan 26, 2012·Cell Communication and Signaling : CCS·Cora S ThielOliver Ullrich
Jan 5, 2012·PloS One·Yulan WangHaiying Hang
Feb 5, 2008·FEBS Letters·Walter MalorniFlavia Franconi
Mar 21, 2015·Journal of Molecular and Cellular Cardiology·Bin ZhengJin-kun Wen
Sep 13, 2014·BioMed Research International·Maria Grazia MasielloMariano Bizzarri
Jul 15, 2017·In Vitro Cellular & Developmental Biology. Animal·Jiao ZhaoZhen Li
Nov 19, 2011·Journal of Applied Physiology·Fabrice DabertrandJean-Luc Morel
Sep 29, 2019·International Journal of Molecular Sciences·Hiroko IkedaAkihisa Takahashi
Oct 9, 2020·International Journal of Molecular Sciences·Mohamed Zakaria NassefMarcus Krüger
Dec 16, 2020·International Journal of Molecular Sciences·Binod PrasadMarcus Krüger

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.