PMID: 2124161Sep 3, 1990Paper

Exposure to N-methyl-D-aspartate increases release of arachidonic acid in primary cultures of rat hippocampal neurons and not in astrocytes

Brain Research
C SanfeliuA J Patel

Abstract

The release of [3H]arachidonic acid (ARA) was investigated from prelabelled primary cultures of hippocampal neurons and astroglial cells. The activation of N-methyl-D-aspartate (NMDA) subtype of glutamate receptors resulted in a dose-dependent stimulation of [3H]ARA release. The half maximal effect was obtained at about 15 microM NMDA, whereas the maximum concentration (50 microM NMDA) produced about a 2-fold increase in 7-day-old cultures. This elevation in [3H]ARA release was blocked in a dose-related manner by the NMDA receptor antagonist, 2-amino-5-phosphonovaleric acid (APV), and by Mg2+ which blocks NMDA receptor-linked Ca2+ ion channels. The removal of external Ca2+ inhibited NMDA-induced release, whereas treatment with calcimycin (A 23187, a Ca2+ ionophore) greatly increased the [3H]ARA release. The inhibitors of phospholipase A2, nordihydroguaiaretic acid and mepacrine, decreased the NMDA-dependent [3H]ARA release in a dose-related manner, maximum inhibition reaching to about 90% at high doses. Entry of Ca2+ brought about by opening the voltage-sensitive channels by high K+ had no effect on the release of [3H]ARA, indicating that NMDA gated channels are situated in a part of the neuron where Ca2+ entry through this rou...Continue Reading

References

May 13, 1977·Brain Research·G A Banker, W M Cowan
May 22, 1985·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·S G Cull-Candy, D C Ogden
Feb 1, 1986·Annals of Neurology·S M Rothman, J W Olney
Jan 2, 1987·Science·R J Miller
Jan 1, 1988·Annual Review of Neuroscience·C W CotmanA H Ganong
Jan 1, 1989·Annals of the New York Academy of Sciences·A I Barkai, L R Murthy
Apr 1, 1989·Current Opinion in Cell Biology·R H Michell
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I LlanoB H Gähwiler
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B R ConklinJ Axelrod
Jan 1, 1987·Progress in Neurobiology·M L Mayer, G L Westbrook
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S N Murphy, R J Miller
Jan 15, 1986·The Biochemical Journal·J L Gordon
Jan 1, 1986·Annual Review of Biochemistry·P NeedlemanJ B Lefkowith
Apr 1, 1987·Journal of Neurochemistry·S K Fisher, B W Agranoff
Jan 1, 1987·Annual Review of Neuroscience·T J Teyler, P DiScenna
Oct 1, 1987·Journal of Neurochemistry·M ReichmanL E Hokin
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Aug 14, 1987·Biochemical and Biophysical Research Communications·M ReichmanL E Hokin
Apr 15, 1982·The Biochemical Journal·R F Irvine
Jan 1, 1983·Neurochemistry International·J W LazarewiczA Y Sun

❮ Previous
Next ❯

Citations

Sep 1, 1994·Molecular and Chemical Neuropathology·E J MurphyL A Horrocks
Jun 1, 1995·Molecular and Chemical Neuropathology·A A FarooquiL A Horrocks
May 1, 1995·Neurochemistry International·N G Bazan, E B Rodriguez de Turco
Aug 1, 1995·Progress in Neurobiology·H Katsuki, S Okuda
Sep 26, 1997·Journal of Lipid Mediators and Cell Signalling·J V Bonventre
Jun 1, 1992·Current Opinion in Neurobiology·Z I Bashir, G L Collingridge
Jan 24, 2004·Brain Research. Brain Research Reviews·John W Phillis, Michael H O'Regan
May 1, 1994·Behavioral and Neural Biology·C Hölscher, S P Rose
Jul 1, 1997·Brain Research. Molecular Brain Research·H A Rohan de SilvaA J Patel
Jun 1, 1997·Neurochemistry International·A A FarooquiL Horrocks
Jul 1, 1997·Journal of Lipid Mediators and Cell Signalling·J V Bonventre
Nov 18, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Mireille BasselinStanley I Rapoport

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.