Exposure to runoff from coal-tar-sealed pavement induces genotoxicity and impairment of DNA repair capacity in the RTL-W1 fish liver cell line

The Science of the Total Environment
Aude KienzlerS Bony

Abstract

Coal-tar-based (CTB) sealcoat, frequently applied to parking lots and driveways in North America, contains elevated concentrations of polycyclic aromatic hydrocarbons (PAHs) and related compounds. The RTL-W1 fish liver cell line was used to investigate two endpoints (genotoxicity and DNA-repair-capacity impairment) associated with exposure to runoff from asphalt pavement with CTB sealcoat or with an asphalt-based sealcoat hypothesized to contain about 7% CTB sealcoat (AS-blend). Genotoxic potential was assessed by the Formamido pyrimidine glycosylase (Fpg)-modified comet assay for 1:10 and 1:100 dilutions of runoff samples collected from 5 h to 36 d following sealcoat application. DNA-repair capacity was assessed by the base excision repair comet assay for 1:10 dilution of samples collected 26 h and 36 d following application. Both assays were run with and without co-exposure to ultraviolet-A radiation (UVA). With co-exposure to UVA, genotoxic effects were significant for both dilutions of CTB runoff for three of four sample times, and for some samples of AS-blend runoff. Base excision repair was significantly impaired for CTB runoff both with and without UVA exposure, and for AS-blend runoff only in the absence of UVA. This st...Continue Reading

References

Dec 1, 1994·Environmental Health Perspectives·J E SteinU Varanasi
Jul 10, 1999·Archives of Environmental Contamination and Toxicology·V A McFarlandD D McCant
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Aug 13, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M BabínJ M Navas
Mar 25, 2006·Ecotoxicology·Pamela J BryerEmily J Willingham
Jan 15, 2009·Cell Biology and Toxicology·Rémi ThibautCinta Porte
Aug 27, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Kevin C RoseJanet M Fischer
May 5, 2010·Ecotoxicology·Thomas BommaritoRichard S Halbrook
Feb 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K SchirmerN C Bols
Aug 11, 2010·Chemosphere·Thomas BommaritoRichard S Halbrook
Jan 19, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·F GagnéG Bickerton
Jan 28, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Aude KienzlerSylvie Bony
Feb 3, 2012·Environmental Science & Technology·Barbara J MahlerE Spencer Williams

❮ Previous
Next ❯

Citations

Dec 15, 2015·Environmental Science & Technology·Jenifer McIntyreNathaniel Scholz
Jul 31, 2016·Environmental and Molecular Mutagenesis·Alexandra S LongPaul A White
Nov 25, 2016·Environmental Toxicology and Chemistry·Austin K BaldwinMatthew Magruder
Aug 31, 2019·Integrated Environmental Assessment and Management·Susan Kane DriscollSolmaz Marzooghi
Jan 12, 2021·Ecotoxicology and Environmental Safety·Helene InderbergÅse Krøkje
Jul 1, 2019·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Bojana Žegura, Metka Filipič

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.