Exposure to Yeast Shapes the Intestinal Bacterial Community Assembly in Zebrafish Larvae

Frontiers in Microbiology
Prabhugouda SiriyappagouderViswanath Kiron

Abstract

Establishment of the early-life gut microbiota has a large influence on host development and succession of microbial composition in later life stages. The effect of commensal yeasts - which are known to create a conducive environment for beneficial bacteria - on the structure and diversity of fish gut microbiota still remains unexplored. The present study examined the intestinal bacterial community of zebrafish (Danio rerio) larvae exposed to two fish-derived yeasts by sequencing the V4 hypervariable region of bacterial 16S rRNA. The first stage of the experiment (until 7 days post-fertilization) was performed in cell culture flasks under sterile and conventional conditions for germ-free (GF) and conventionally raised (CR) larvae, respectively. The second phase was carried out under standard rearing conditions, for both groups. Exposure of GF and CR zebrafish larvae to one of the yeast species Debaryomyces or Pseudozyma affected the bacterial composition. Exposure to Debaryomyces resulted in a significantly higher abundance of core bacteria. The difference was mainly due to shifts in relative abundance of taxa belonging to the phylum Proteobacteria. In Debaryomyces-exposed CR larvae, the significantly enriched taxa included ben...Continue Reading

References

Jun 29, 1999·Microbial Ecology·G H Hansen, J A Olafsen
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·L VerschuereW Verstraete
May 16, 2001·Science·L V Hooper, J I Gordon
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M W CalfeeE C Pesci
Nov 24, 2004·Molecular Microbiology·Deborah A HoganRoberto Kolter
Dec 8, 2005·Applied and Environmental Microbiology·Catherine Lozupone, Rob Knight
Jul 21, 2007·Molecular Microbiology·Carla CuginiDeborah A Hogan
May 24, 2008·Science·Ruth E LeyJeffrey I Gordon
Jan 1, 2008·Interdisciplinary Perspectives on Infectious Diseases·Paul W O'Toole, Jakki C Cooney
Dec 1, 2009·Nature Reviews. Microbiology·Michael E HibbingS Brook Peterson
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Apr 8, 2011·The ISME Journal·Guus RoeselersJohn F Rawls
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Jan 10, 2012·Annual Review of Food Science and Technology·Petra A M J ScholtensJan Knol
Mar 21, 2012·Zebrafish·Leon CantasHenning Sørum
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jorge Galindo-VillegasVictoriano Mulero
Dec 26, 2012·Frontiers in Microbiology·Rima HatoumIsmail Fliss
Jun 13, 2013·Current Opinion in Microbiology·Iliyan D Iliev, David M Underhill
Jan 1, 2012·ISRN Microbiology·Patricia Martínez CruzHugo C Ramírez Saad
Jul 6, 2013·Science·Jeremiah J FaithJeffrey I Gordon
Aug 8, 2013·PloS One·Eric J de MuinckPål Trosvik
Oct 15, 2014·Journal of Pediatric Gastroenterology and Nutrition·Pearl D Houghteling, W Allan Walker
Feb 6, 2015·Microbial Ecology in Health and Disease·Juan Miguel RodríguezMaria Carmen Collado
Jun 30, 2015·Journal of Applied Microbiology·N V Hai
Jul 4, 2015·Bioinformatics·Robert C Edgar, Henrik Flyvbjerg
Aug 6, 2015·Cellular Microbiology·Marios Arvanitis, Eleftherios Mylonakis
Sep 5, 2015·The ISME Journal·W Zac StephensBrendan J M Bohannan
Oct 27, 2015·Frontiers in Immunology·Ana Montalban-ArquesJorge Galindo-Villegas
Jan 6, 2016·Scientific Reports·Silvia FalcinelliOliana Carnevali
Oct 30, 2016·The Veterinary Quarterly·Mohammad Jalil ZorriehzahraCarlo C Lazado
May 7, 2016·The ISME Journal·Sheerli Kruger Ben ShabatItzhak Mizrahi
May 25, 2016·Behavioural Brain Research·Daniel J DavisAaron C Ericsson
Jan 11, 2017·The ISME Journal·Alexandre JoussetW H Gera Hol
Aug 23, 2017·Allergology International : Official Journal of the Japanese Society of Allergology·Masaru Tanaka, Jiro Nakayama

❮ Previous
Next ❯

Citations

Mar 3, 2020·Frontiers in Immunology·Adrià López NadalSylvia Brugman
Jun 13, 2020·Frontiers in Immunology·Prabhugouda SiriyappagouderJorge M O Fernandes
Feb 9, 2019·Frontiers in Endocrinology·Robyn Lisa Butt, Helene Volkoff

❮ Previous
Next ❯

Datasets Mentioned

BETA
ERS2484299
ERS2484358
CBS8339

Methods Mentioned

BETA
PCR
dissection
Assay

Software Mentioned

QIIME
ggplot2
phyloseq
ANOSIM
FastQC
iNEXT
Adonis
USEARCH
xlsx
RStudio

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.