Exposure to Zinc oxide nanoparticles during pregnancy induces oocyte DNA damage and affects ovarian reserve of mouse offspring

Aging
Qiu-Yue ZhaiWei Shen

Abstract

Zinc oxide nanoparticles (nZnO) have been shown to have higher toxic effects likely due to their ion-shedding ability and low solubility under neutral conditions. In order to investigate whether exposure to nZnO during embryonic development affects ovary development, 12.5 day post coitum (dpc) fetal mouse ovaries were cultured in the presence of nZnO for 6 days. We found that the nanoparticles (NPs) accumulated within the oocyte cytoplasm in a dose dependent manner, caused DNA damage and apoptosis, and result in a significant decrease in oocyte numbers. No such effects were observed when the ovaries were incubated in the presence of ZnSO4 or bulk ZnO as controls. In addition, we injected intravenously 16 mg/kg body weight nZnO in 12.5 dpc pregnant mice on two consecutive days and analyzed the ovaries of fetuses or offspring at three critical periods of oogenesis: 17.5 dpc, 3 days post-partum (dpp) and 21 dpp. Evidence of increased DNA damage in pachytene oocytes in fetal ovaries and impaired primordial follicle assembly and folliculogenesis dynamics in the ovaries of the offspring were found. Our results indicate that certain types of NPs affect pre- and post-natal oogenesis in vitro and in vivo.

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Citations

Mar 19, 2020·Critical Reviews in Toxicology·S Keerthana, A Kumar
Jan 17, 2019·Journal of Agricultural and Food Chemistry·Tian-Yu ZhangWei Shen
Jun 8, 2021·Reproductive Toxicology·Ramsés Santacruz-MárquezIsabel Hernández-Ochoa
Oct 26, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Maryam SaberFereshteh Esfandiari
Jul 9, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Bolu ChenHengyi Xu
Sep 24, 2021·Molecular Reproduction and Development·José Roberto Viana SilvaRicardo Pires Dos Santos

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Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad Prism
Leica Laser Scanning Confocal Microscope
GraphPad

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