Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge

Marine Drugs
Christine KlingerH Ehrlich

Abstract

Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida are known to contain both chitin and biologically active bromotyrosines. Aplysina archeri (Aplysineidae: Verongiida) is well known to contain bromotyrosines with relevant bioactivity against human and animal diseases. The aim of this study was to develop an express method for the production of naturally prefabricated 3D chitin and bromotyrosine-containing extracts simultaneously. This new method is based on microwave irradiation (MWI) together with stepwise treatment using 1% sodium hydroxide, 20% acetic acid, and 30% hydrogen peroxide. This approach, which takes up to 1 h, made it possible to isolate chitin from the tube-like skeleton of A. archeri and to demonstrate the presence of this biopolymer in this sponge for the first time. Additionally, this procedure does not deacetylate chitin to chitosan and enables the recovery of ready-to-use 3D chitin scaffolds without destruction of the unique tube-like fibrous interconnected structure of the isolated biomaterial. Furthermore, these mechanically stressed fibers still have the capacity for saturation with water, methylene b...Continue Reading

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Citations

Oct 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tomasz MachałowskiHermann Ehrlich
Sep 27, 2019·Scientific Reports·Judy GopalSechul Chun
Oct 30, 2019·Marine Drugs·Valentine KovalchukHermann Ehrlich
Dec 2, 2020·Marine Drugs·Yuliya KhrunykHermann Ehrlich
Dec 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Saravut Satitsri, Chatchai Muanprasat
Jan 12, 2021·Applied Physics. A, Materials Science & Processing·Liubov MuzychkaHermann Ehrlich
Mar 2, 2021·Biochemistry and Biophysics Reports·Oluwatoosin B A AgbajeDorrit E Jacob
Nov 14, 2020·Carbohydrate Polymers·Mikhail V TsurkanHermann Ehrlich
May 18, 2021·Bioactive Materials·Mei-Chen WanLi-Na Niu
Jul 3, 2021·Marine Drugs·Zhaoyu ZhangSidong Li
Jul 17, 2021·International Journal of Biological Macromolecules·Murugan Prasathkumar, Subramaniam Sadhasivam
Sep 24, 2021·Critical Reviews in Food Science and Nutrition·Esmaeil SheibaniHermann Ehrlich
Mar 10, 2020·Journal of Environmental Management·Tomasz MachałowskiTeofil Jesionowski

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Methods Mentioned

BETA
chemical treatments
scanning electron microscopy
light microscopy
Infrared
infrared spectroscopy
X-ray

Software Mentioned

OMNIC Lite
MATLAB
OriginLab
OriginPro

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