PMID: 8603078Mar 13, 1996Paper

Expressed human hippocampal ASCT1 amino acid transporter exhibits a pH-dependent change in substrate specificity

Biochimica Et Biophysica Acta
B K TamarappooM S Kilberg

Abstract

In mammalian cells, the basal Na+-dependent uptake for many of the neutral amino acids is mediated by a transport activity designated System ASC. A cloned human brain cDNA sequence, ASCT1, encodes a Na+-dependent neutral amino acid transport activity that exhibits a substrate specificity similar to that commonly associated with System ASC. However, the characteristics of ASC activity varies significantly between cell types and not all tissues contain detectable levels of ASCT1 mRNA. A unique property of System ASC activity is an altered substrate selectivity such that at pH values below 7.4 anionic amino acids function as inhibitors and substrates. The experiments in this report were designed to determine if the cloned ASCT1 transporter exhibited this pH-dependent anionic transport. Following transfection of HeLa cells with the ASCT1 cDNA, transport strongly favored neutral zwitterionic) amino acids when uptake was measured at a physiologic pH value of 7.5. However, lowering the assay pH to 5.5 significantly enhanced the interaction of the ASCT1 carrier with anionic amino acids such as cysteate, in a pH-dependent manner. The apparent pK for the titratable group was in the range of 6.5-7.0. These results provide evidence that th...Continue Reading

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Citations

Nov 13, 2003·European Journal of Pharmacology·Yoshikatsu Kanai, Matthias A Hediger
Aug 14, 2003·Brain Research·Takeshi TakaradaYukio Yoneda
Oct 25, 2001·Brain Research. Developmental Brain Research·M D WeissK J Anderson
May 2, 2001·Journal of Neurochemistry·S Bröer, N Brookes
Nov 19, 2003·Journal of Neurochemistry·Kazuhiro TetsukaTetsuya Terasaki
Feb 18, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Feb 5, 2002·Canadian Journal of Physiology and Pharmacology·J PinillaM P Lostao
Dec 10, 2015·British Journal of Pharmacology·Stephen Ph AlexanderUNKNOWN CGTP Collaborators
Nov 8, 1996·The Journal of Biological Chemistry·N Zerangue, M P Kavanaugh
Oct 28, 1998·Physiological Reviews·M PalacínA Zorzano
Nov 6, 2018·Molecular Cancer Research : MCR·Mark A White, Daniel E Frigo
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·D J SlotboomJ S Lolkema
Jul 17, 2021·SLAS Discovery·Mariafrancesca ScaliseCesare Indiveri

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