Expressed sequence tag analysis and development of gene associated markers in a near-isogenic plant system of Eragrostis curvula

Plant Molecular Biology
Gerardo D L CervigniViviana C Echenique

Abstract

Eragrostis curvula (Schrad.) Nees is a forage grass native to the semiarid regions of Southern Africa, which reproduces mainly by pseudogamous diplosporous apomixis. A collection of ESTs was generated from four cDNA libraries, three of them obtained from panicles of near-isogenic lines with different ploidy levels and reproductive modes, and one obtained from 12 days-old plant leaves. A total of 12,295 high-quality ESTs were clustered and assembled, rendering 8,864 unigenes, including 1,490 contigs and 7,394 singletons, with a genome coverage of 22%. A total of 7,029 (79.11%) unigenes were functionally categorized by BLASTX analysis against sequences deposited in public databases, but only 37.80% could be classified according to Gene Ontology. Sequence comparison against the cereals genes indexes (GI) revealed 50% significant hits. A total of 254 EST-SSRs were detected from 219 singletons and 35 from contigs. Di- and tri- motifs were similarly represented with percentages of 38.95 and 40.16%, respectively. In addition, 190 SNPs and Indels were detected in 18 contigs generated from 3 to 4 libraries. The ESTs and the molecular markers obtained in this study will provide valuable resources for a wide range of applications includin...Continue Reading

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Citations

Mar 12, 2011·Sexual Plant Reproduction·Ana Claudia OchogavíaSilvina Claudia Pessino
Mar 4, 2008·Plant Molecular Biology·Gerardo D L CervigniViviana Echenique
Jan 23, 2009·Plant Molecular Biology·Ana C OchogavíaSilvina C Pessino
Jun 21, 2011·Journal of Plant Physiology·Jiyu ZhangZhibiao Nan
Jul 30, 2019·Frontiers in Plant Science·Diego ZappacostaViviana Echenique
Nov 9, 2018·Frontiers in Plant Science·Micaela ManciniSilvina C Pessino
Nov 11, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Diego Hojsgaard, Manfred Schartl

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