Expressed sequence tag (EST) analysis of the erythrocytic stages of Babesia bovis

Veterinary Parasitology
Erik de VriesMatthew Berriman

Abstract

Expressed sequence tags (ESTs) provide an efficient way to identify large numbers of genes expressed in a specific stage of the life cycle of an organism. Here we analysed approximately 13,000 ESTs derived from the erythrocytic stage of the apicomplexan parasite Babesia bovis. The ESTs were clustered in order to obtain information on the expression level of a gene and to increase sequence length and reliability. A total of 3522 clusters were obtained and annotated using BLAST algorithms. The clusters were estimated to represent approximately 2600 genes of which in total approximately 2.1 Mbp sequence information was obtained. Expression levels of the genes, as determined by the numbers of ESTs contained within a cluster, were compared to those of their closest homologs in the erythrocytic stage of Plasmodium falciparum and Toxoplasma gondii tachyzoites. Pathways that are represented relatively abundant in B. bovis are, amongst others, the purine salvage pathway (displaying characteristics not identified before in apicomplexans), isoprenoid biosynthesis in the apicoplast and many genes encoding mitochondrial proteins. Especially remarkable in the latter group are the F-type ATPases - which are hardly expressed in P. falciparum a...Continue Reading

References

May 1, 1992·Molecular and Biochemical Parasitology·J E Feagin
Jan 1, 1990·Parasitology Research·C MatiasA M Gero
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 1, 1995·Molecular and Biochemical Parasitology·S A HinesA C Rice-Ficht
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·A A Escalante, F J Ayala
Jul 1, 1993·Molecular and Biochemical Parasitology·D R AllredK P Ahrens
Jul 31, 1998·Annals of Tropical Medicine and Parasitology·I A Clark, L S Jacobson
Dec 10, 1998·International Journal for Parasitology·N Lang-UnnaschJ R Barta
Jun 23, 1999·Parasitology Today·W C Brown, G H Palmer
Oct 16, 1999·European Journal of Biochemistry·C CarretA Gorenflot
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R M O'Connor, D R Allred
Mar 28, 2002·Journal of Molecular Biology·Detlef D LeipeL Aravind
Jun 8, 2002·Journal of Molecular Biology·R J M Iain Wilson
Oct 9, 2002·Nature·Malcolm J GardnerBart Barrell
Feb 1, 2003·Science·Bernardo J FothGeoffrey I McFadden
Jan 30, 2004·Molecular and Biochemical Parasitology·David R Allred, Basima Al-Khedery
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Boris StriepenJessica C Kissinger
Feb 24, 2004·Molecular and Cellular Biochemistry·Michel RigouletLena Gustafsson

❮ Previous
Next ❯

Citations

Jul 30, 2014·Parasitology·Monica Florin-ChristensenLeonhard Schnittger
Oct 24, 2007·PLoS Pathogens·Kelly A BraytonVishvanath M Nene
Jul 9, 2011·PLoS Neglected Tropical Diseases·Won Gi YooSung-Jong Hong
Mar 17, 2011·Molecular and Biochemical Parasitology·Mohamad Alaa TerkawiIkuo Igarashi
Jul 6, 2010·Trends in Parasitology·Sejal GohilBrian M Cooke
Nov 10, 2009·Veterinary Parasitology·Mahmoud AboulailaIkuo Igarashi
Mar 11, 2008·Veterinary Parasitology·Tiago M MartinsAna Domingos
Jul 28, 2007·Trends in Parasitology·Kirsty JensenElizabeth J Glass
Apr 20, 2007·Experimental Parasitology·Audrey O T LauTerry F McElwain
Jun 12, 2010·Communicative & Integrative Biology·Björn F C Kafsack, Vern B Carruthers
May 27, 2011·Veterinary Parasitology·Hoyam AwadAbdelrahim M El Hussein
Jul 22, 2008·Molecular and Biochemical Parasitology·Elvira T DerbyshireHenry M Staines
Oct 17, 2012·International Journal for Parasitology·Sejal GohilBrian M Cooke
Jan 26, 2017·The FEBS Journal·Betsaida Bibo-VerdugoAnthony J O'Donoghue
Nov 8, 2017·Parasites & Vectors·Azirwan GuswantoIkuo Igarashi
Feb 21, 2019·MBio·Lee M YeohStuart A Ralph
Aug 16, 2014·BMC Genomics·Junya YamagishiIkuo Igarashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.

Babesiosis (ASM)

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.